Claim · model-written summary
Barley alpha-amylase antibodies cross-reacted with all Araucaria alpha-amylases.
- Taxon
- Araucaria araucana
Araucaria araucanae1 - Gene
- A . araucana α‐amylasese0
- Outcome
- cross‐reacted with all the A . araucana α‐amylasese0
Verbatim
e0“polyclonal antibodies generated against barley α‐amylase cross‐reacted with all the A . araucana α‐amylases.”
e1“Araucaria araucana”
Title
Biochemical and immunological characterization of α‐amylase isoenzymes of Araucaria araucana
Elba Acevedo; Liliana Cardemil Physiologia Plantarum 1994. doi:10.1111/j.1399-3054.1994.tb06666.x
Other claims from this paper (8)
- Observation Seven alpha-amylase isoenzymes were purified from quiescent Araucaria seeds.
- Observation The seven isoenzymes had masses of 45.7, 47.0, 50.2, 51.2, 52.0, 53.5 and 55.2 kDa.
- Method / resource The two main isoforms were separated chromatographically from each other and other isoenzymes.
- Observation All isoenzyme bands stained with periodic acid/dansylhydrazine, suggesting glycoprotein status.
- Observation The isoenzymes are rich in glycine, aspartic acid/asparagine, alanine, serine, proline and glutamic acid/glutamine.
- Observation The three sequenced isoenzymes had identical N-terminal sequences.
- Observation Composition and N-terminal sequence comparisons found no relation to cereal and Vigna radiata alpha-amylases.
- Observation Peptide maps suggested genetic differences among Araucaria isoenzymes.