Claim · model-written summary
A two-nucleotide deletion in exon-2 of the A copy leads to a premature stop codon, suggesting a non-functional protein.
Triticum aestivumVerbatim
e2“two nucleotides deleted in exon-2 of the A copy lead to a premature stop codon and suggest that it encodes a non-functional protein”
t0“in bread wheat (Triticum aestivum L.)”
Title
Fatiha Benyahya; Isabelle Nadaud; Olivier Da Ines; et al. The Plant Journal 2020. doi:10.1111/tpj.14903
Other claims from this paper (10)
- Observation The three SPO11-2 copies of hexaploid wheat show high nucleotide and amino acid similarity to those of diploids, tetraploids and Arabidopsis.
- Observation The mutation was absent from Triticum urartu.
- Observation The mutation was present in Triticum dicoccoides and in different wheat cultivars.
- Observation The mutation occurred after the first polyploidy event and has been conserved since.
- Intervention effect Triple mutants with all three SPO11-2 copies (A, B, D) inactivated are sterile.
- Intervention effect Double mutants retaining only the A copy are also sterile.
- Observation Some DMC1 foci remain visible in the A-copy-only double mutant, suggesting residual activity of the A copy that is not sufficient for fertility.
- Intervention effect Triple mutants show severe reductions in bivalent formation and numbers of DMC1 foci.
- Intervention effect Triple mutants show synapsis defects.
- Mechanism TaSPO11-2 has an essential role in meiotic recombination in wheat.