Claim · model-written summary
Oidiodendron griseum DNA could not be detected at any concentration when combined with DNA from S9 or S246, indicating a PCR bias against it.
- Interactor
- O. griseumOidiodendron griseume0symbiont
- Outcome
- O. griseum. DNA could not be detected at any concentratione0
- Comparator
- DNA from either S9 or S246e0
Verbatim
e0“The O. griseum. DNA could not be detected at any concentration when it was combined with DNA from either S9 or S246”
Title
Studies of interactions among cultured ericoid mycorrhizal fungi of salal (gaultheria shallon pursh)
Nafar, Firoozeh cIRcle (University of British Columbia) 2009. doi:10.14288/1.0088467
Other claims from this paper (10)
- Mechanism The PCR bias explained why O. griseum never appeared in RFLP patterns from amplified DNA of synthesized salal mycorrhizae.
- Observation O. griseum was re-isolated from 71% of the root segments of synthesized salal mycorrhizae.
- Method / resource RFLPs clearly indicated mixtures of the two sterile isolates, which were difficult to recognize by morphology.
- Observation Two mycorrhizal fungi often occupied the same 5 mm segment of a salal root.
- Observation Where roots lay under both inocula, 70% of cultures recovered were mixed cultures when a sporulating and a sterile fungus were used as inoculum.
- Observation All pairings of the three ericoid mycorrhizal fungi in culture showed deadlock: neither species could invade the territory of the other.
- Observation Synthesized salal mycorrhizae formed after three to four months.
- Intervention effect Replacing granulated agar with phytagel improved the clarity of the gel.
- Intervention effect Phytagel significantly improved the shoot growth of salal compared with granulated agar.
- Intervention effect Percent root colonization of salal showed no significant difference between phytagel and granulated agar.
Gaultheria shallon