Claim · model-written summary
The Doyle and Doyle CTAB method produced higher DNA concentration from L balsamina.
- Taxon
- L balsaminae0
- Treatment
- CTAB method of Doyle and Doylee0
- Outcome
- DNA concentratione0
- Direction
- highere0
Not reportedComparator
Verbatim
e0“The CTAB method of Doyle and Doyle produced higher DNA concentration from L balsamina, most probably due to the presence of ammonium acetate in the washing buffer and longer incubation time (2 h).”
Title
Farah Izana Abdullah; Lee Suan Chua; Zaidah Rahmat; et al. Natural Product Communications 2016. doi:10.1177/1934578x1601101017
Other claims from this paper (6)
- Mechanism Ammonium acetate and longer incubation probably explain the higher DNA concentration from L balsamina.
- Method / resource All modified CTAB methods obtained high DNA concentration of acceptable quality from A. paniculata.
- Method / resource Ο. aristatus had likely lower DNA concentration of 33-87 μg/g and quality.
- Mechanism High phenolic compounds, particularly rosmarinic acid, probably explain lower DNA concentration and quality in Ο. aristatus.
- Method / resource Extracted genomic DNA was effectively amplified with ITS primers AB 101 and AB 102 at 48°C.
- Method / resource The DNA sequences had high similarity to ITS sequences of similar plant species in GenBank.