Abstract
SummaryChinese apricot (Prunus armeniaca L.) shows gametophytic self-incompatibility (GSI) controlled by a single locus containing at least two linked genes [i.e., the S-RNase gene and the pollen-expressed SFB (or SLF) gene] as do other fruit species in the family, Rosaceae. To elucidate the S-genotypes of 14 native Chinese apricot cultivars, PCR was performed using primers designed from Prunus S-RNase and SFB consensus sequences. After cloning and sequencing the PCR products, the S-genotypes of all 14 apricot cultivars were determined, and eight new S-RNase alleles and nine SFB alleles were identified. The S-RNases shared typical structural features with S-RNases from other Prunus spp. exhibiting GSI. The SFB genes showed similar structural characteristics to SFB genes in other Prunus spp. The intron sequences of the SFB genes revealed sequence and length polymorphisms. The deduced level of amino acid sequence identity for the eight new S-RNase alleles was 66.4 – 100% in P. armeniaca, while the similarity of the SFB alleles was 73.7 – 98.6%. The physical distances between the SFB and S-RNase genes was determined exactly in the S9,S11,S17, and S26-haplotypes, confirming that the S-RNase and SFB genes were linked. The range of distances between the two genes was 299 – 1,061 bp. This study increases our knowledge on the S-genotypes of apricot native to China, and enriches our genomic information on GSI in the Prunus genus.
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Claims 10
Claim · model-written summary
S genotypes were determined for all 14 apricot cultivars.
- Taxon
- apricot
Prunus armeniacae0 - Outcome
- S-genotypese0
- Value
- 14 apricot cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the S-genotypes of all 14 apricot cultivars were determined”
Claim · model-written summary
Eight new S-RNase alleles were identified.
- Taxon
- apricotPrunus armeniacat0
- Gene
- S-RNasee0
- Outcome
- S-RNase allelese0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight new S-RNase alleles and nine SFB alleles were identified”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
Nine SFB alleles were identified.
- Taxon
- apricotPrunus armeniacat0
- Gene
- SFBe0
- Outcome
- SFB allelese0
- Value
- ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight new S-RNase alleles and nine SFB alleles were identified”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
Apricot S-RNases shared typical features with other Prunus S-RNases.
- Taxon
- apricotPrunus armeniacat0
- Gene
- S-RNasese0
- Outcome
- typical structural featurese0
- Comparator
- other Prunus spp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The S-RNases shared typical structural features with S-RNases from other Prunus spp. exhibiting GSI.”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
Apricot SFB genes had structures similar to other Prunus SFB genes.
- Taxon
- apricotPrunus armeniacat0
- Gene
- SFB genese0
- Outcome
- similar structural characteristicse0
- Comparator
- other Prunus spp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SFB genes showed similar structural characteristics to SFB genes in other Prunus spp.”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
SFB introns varied in sequence and length.
- Taxon
- apricotPrunus armeniacat0
- Gene
- SFB genese0
- Outcome
- sequence and length polymorphismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The intron sequences of the SFB genes revealed sequence and length polymorphisms.”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
New S-RNase allele amino acid identities ranged from 66.4 to 100%.
- Taxon
- P. armeniacaPrunus armeniacae0
- Gene
- S-RNasee0
- Outcome
- amino acid sequence identitye0
- Value
- 66.4 – 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amino acid sequence identity for the eight new S-RNase alleles was 66.4 – 100% in P. armeniaca”
Claim · model-written summary
SFB allele similarities ranged from 73.7 to 98.6%.
- Taxon
- apricotPrunus armeniacat0
- Gene
- SFBe0
- Outcome
- similaritye0
- Value
- 73.7 – 98.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“similarity of the SFB alleles was 73.7 – 98.6%”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
Physical mapping confirmed S-RNase and SFB linkage.
- Taxon
- apricotPrunus armeniacat0
- Gene
- S-RNase and SFB genese0
- Outcome
- were linkede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confirming that the S-RNase and SFB genes were linked”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
Claim · model-written summary
The intergene distance ranged from 299 to 1,061 bp.
- Taxon
- apricotPrunus armeniacat0
- Gene
- SFB and S-RNase genese1
- Outcome
- distances between the two genese0
- Value
- 299 – 1,061 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The range of distances between the two genes was 299 – 1,061 bp.”
t0“Identification ofS-haplotype-specificS-RNaseandSFBalleles in native Chinese apricot (Prunus armeniacaL.)”
e1“The physical distances between the SFB and S-RNase genes was determined exactly”