Abstract
The in vitro antibiotic tests of 26 Chinese herbal medicines,such as Rhizoma coptidis,Radix scutellariae,Herba taraxaci and Herba violae,etc.were conducted on the isolated Staphylococcus aureus and Escherichia coli from the dairy cow with subclinical mastitis by cylinder plate and microdilution methods.The result of the plate method test indicated that the Rhizoma coptidis,Radix scutellariae,Herba violae,Radix isatidis etc.were more effective compared with other Chinese herbal medicines on the S.aureus and E.coli,and their sensitivity to the 2 pathogens are moderate or highly,or extremely high,the range of antibacterial diameters was 19.00-24.93 mm.The result of the microdilution method test also showed that the Rhizoma coptidis,Radix scutellariae,Flos carthami,Flos lonicerae,Radix isatidis,etc.were more effective compared with other Chinese herbal medicines on the S.aureus and E.coli,their MIC was 0.004-0.063 g/mL,and their MBC was 0.004-0.063 g/mL respectively.This study provides a scientific basis for the development of traditional Chinese medicines on the external treatments of the mastitis in dairy cow.
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