Abstract
2 Abstract: In this study eight antibiotics and six heavy metals were used for genetic marking of seven Rhizobium strains and six Pseudomonas strains to be used in conjugation. Six pseudomonas strains were selected on the basis of IAA and salicylic acid production to be used as a donor against seven Rhizobium strains as recipients. All the matins between rhizobiunm and pseudomonas strains were successed except for the four matings between; RL-3841 X P.putida21, RL-4406 X P.putida21, RL- 4404 X P.putida21 and RL-4404 X PF-10 which did not appeared any recombinants on selective medium. Plasmids from seven rhizobium and six Pseudomonas strains were cured using elevated 12 37 40 45 52 53 78 79 temperatures method. Some recombinants (Tr , Tr , Tr , Tr , Tr , Tr , Tr and Tr ) appeared significant increase in IAA production usim the complete medium supplemented with tryptophan in relation to the mid-parents. All bacterial transconjugants harboring DNA from two sources produced high levels of salicylic, these have a beneficial effect in controlling plant parasites. Bacteriocin produced from all Rhizobium strains inhibit the growth of PF-50090 strain, except for that produced from two strains RL-3841 and RL-2070. In contrast bacteriocins produced from all Rhizobium strains did not inhibit on the growth of P.putida. Bacteriocins produced from P. putida21 inhibit all rhizobium strains. All bacteriocines produced by bacterial strains caused growth inhibition against Agr1513 except for three Rhizobium strains; RL-2070, RL-2074 and RL-207. As well as, all bacteriocins produced by transconjugants had the same effect against Agr1513, except for the 10 transconjugant Tr . All Pseudomonas strains were successfully to inhibit the growth of Agr1513 in Petri dishes. Whereas, only one Rhizobium strain RL-4404 had the same effect against Agr1513 in contrast, the other strains were failed to appear the same effect of antagonism. Six transconjugants 4567 9 14 (Tr , Tr , Tr , Tr , Tr and Tr ) were successfully to appeared antagonism against Agr1513. All Rhizobium strains and their transconjugants appeared significant increase in nodules number per plant above the uninoculated plants among both seasons. Successful nodulation was achieved by the 23578 15 transconjugants Tr , Tr , Tr , Tr , Tr and Tr which produced a higher number of nodules developed 23578 15 per plant. Successful nodulation was achieved by transconjugants: Tr , Tr , Tr , Tr , Tr and Tr which produced a higher number of nodules developed per plant. All bacterial strains play an important role to improve the concentration of chlorophyll a at 45 days plant-old during both seasons and total 1357891 0 1 4 chlorophyll during the first season. Rhizobium transconjugants (T , Tr , Tr , Tr , Tr , Tr , Tr and Tr ) appeared significant increase in shoot N content at 45 days plant-old above uninoculated plants among two season. All rhizobium strains and their transconjugants appeared significant increase in seeds 11 1 content of total protein above uninoculated plants among both seasons except for Tr and Tr at the first season.
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