Abstract
The inheritance of resistance to an Indonesian isolate of powdery mildew in melon cultivar PI 371795 was previously determined and it is controlled by a single dominant gene, to which the symbol Pm-W was assigned. However, the origin of Pm-W gene in PI 371795 and their generations were not characterized. In this study we sought to define the inheritance of Pm-Wgene among resistant cultivars PI 371795, susceptible cultivar Action 434, F1, F2, and Testcross generations by RAPD marker analysis. As the result, a RAPD marker which produced the specific DNA bands linked to PmW was shown only in resistant cultivar of PI 371795, F1 generation, F2 and Testcross resistant. However, the DNA band was not shown in the susceptible cultivars Action 434, F2 and Testcross susceptible, respectively. This result revealed that a specific DNA band linked to Pm-Wgene in melon PI 371795 and their generations was derived from the resistant parental cultivar of PI 371795 Keywords: Melon, powdery mildew, resistance gene, RAPD
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Claims 3
Claim · model-written summary
The Pm-W-linked RAPD band occurred in resistant PI 371795, F1, resistant F2, and resistant Testcross progeny.
- Taxon
- MELON
.jpg?width=60)
Cucumis melot0 - Material
- PI 371795e0
- Gene
- PmWe0
- Outcome
- specific DNA bands linked to PmWe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“a RAPD marker which produced the specific DNA bands linked to PmW was shown only in resistant cultivar of PI 371795, F1 generation, F2 and Testcross resistant”
t0“IN MELON”
Claim · model-written summary
The Pm-W-linked DNA band was absent in susceptible Action 434, F2, and Testcross progeny.
- Taxon
- MELONCucumis melot0
- Material
- Action 434e0
- Outcome
- DNA band was not showne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DNA band was not shown in the susceptible cultivars Action 434, F2 and Testcross susceptible”
t0“IN MELON”
Claim · model-written summary
The Pm-W-linked DNA band in melon PI 371795 generations was derived from resistant PI 371795.
- Taxon
- melonCucumis meloe0
- Material
- PI 371795e0
- Gene
- Pm-Wgenee0
- Outcome
- specific DNA band linked to Pm-Wgenee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a specific DNA band linked to Pm-Wgene in melon PI 371795 and their generations was derived from the resistant parental cultivar of PI 371795”