Abstract
The effect of temperature,pH,light,carbon source,nitrogen source and moisture on the mycelial growth,sporulation and conidial germination of loquat gray leaf spot fungus [Pestalotiopsis eriobotryfolia(Guba) Chen et Chao] was primarily studied.For the mycelia growth of P.eriobotryfolia,the range of temperatures was 8 to 36 ℃,the optimum being 24 ℃;and the range of pH was 3 to 10,the optimum being 5.For the sporulation,the range of temperatures was 8 to 34 ℃,the optimum being 26 ℃;and the range of pH was 3 to 10,the optimum being 4.For the conidial germination,the range of temperature was 8 to 36 ℃,the optimum being 28 ℃;and the range of pH was 3 to 10,the optimum being 5.When the relative humidity reached 100% or in water,the percentage of conidial germination was the highest;the conidia were found not germinated when the RH was below 90%.The maltose,or sucrose could promote the conidial germination.The ammonium sulfate,ammonium phosphate,or ammonium nitrate could inhibit the conidial germination.The lethal temperature for the spores was 50 ℃ maintaining 10 minutes or 55 ℃ maintaining 2 min,while the lethal temperature for the mycelium was 50 ℃ maintaining 15 min or 55 ℃ maintaining 2 min.
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