Abstract
Abstract Medicago truncatula is a model legume species for molecular biology studies, while its close relative M. sativa (alfalfa) is an important crop. Extensive genomic investigations have already resulted in fine genetic maps, physical maps and in many functional genomic tools as well as the sequence information of a large part of the Medicago genome. An initial cytogenetic map of M. truncatula have also been constructed and its present work is focusing on the most telomeric DNA sequences, as well as those most probably targeted to the euchromatic-heterochromatic borders in each chromosome of M. truncatula and using them mapping experiments to reveal the length of the sequenced gene space. In the present work, we used these M. truncatula molecular markers to identify the homologous counterpart from alfalfa using multiplex PCR approach. We have successfully identified 15 DNA sequences from alfalfa that are suitable to tag different chromosomal positions hybridizing experiments and contribute to the drawing of alfalfa cytogenetic map.
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Claims 1
Claim · model-written summary
Fifteen alfalfa DNA sequences were identified as suitable chromosomal position tags.
- Taxon
- alfalfa
Medicago sativae0 - Outcome
- DNA sequencese0
- Value
- 15e0
- Qualifier
- suitable to tag different chromosomal positionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have successfully identified 15 DNA sequences from alfalfa that are suitable to tag different chromosomal positions hybridizing experiments”