Abstract
During the present study, 6 deletion lines (del 5AS-3, del5AS-7, del5AS-10, del5AL-10, del5AL-12 and del5AL-23) of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 5A and one deletion line (5DS-1) used as a positive control. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism for long arm deletion line del5AL-23. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 5A.
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Claims 2
Claim · model-written summary
Primer OPA-07 showed useful polymorphism for the long-arm deletion line del5AL-23 among seven RAPD primers.
- Taxon
- common wheat
Triticum aestivume1 - Material
- del5AL-23e2
- Outcome
- polymorphisme2
- Comparator
- 7 RAPD primerse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers”
e2“Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism for long arm deletion line del5AL-23”
Claim · model-written summary
Primer OPA-07 anneals to the distal half of the long arm of chromosome 5A.
- Taxon
- common wheatTriticum aestivume1
- Outcome
- anneals to the distal half of the long arm of chromosome 5Ae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers”
e3“it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 5A”