Abstract
Eighteen strains of Root nodule bacteria were collected from the chickpea plant, grown in different areas of middle Gujarat, viz., Anand, Dahod, Thasara, Arnej and Dhanduka. These strains were confirmed as Rhizobium by using different biochemical test and maintained their pure culture. Molecular characterization based on repetitive DNA sequence especially, ERIC sequence (Enterobacterial Repetitive Intergeneric Consensus) were done together with two known Rhizobium strains, one commercial culture (GSFC, Vadodara), five standard strains of Rhizobium and one standard strain of Agribacterium tumefacinus. The total of 320 no of amplicons was generated by using ERIC primer pair. The strain MTCC 4188 (Mesorhizobium ciceri) produced highest no of amplicons while strain MTCC 120 (Bradyrhizobium japonicum) showed a less no of amplicons. Data analysis of ERIC fingerprinting pattern clustered all RNB strains and standard strains into four major clusters as per their phylogenetic relationship. Majority of RNB strains (65 per cent) were closely related to the genus Mesorhizobium ciceri species and Mesorhizobium loti, while remaining 40 per cent RNB strains showed similarity to Rhizobium leguminosarum (MTCC 99) and Agrobacterium tumefaciens (MTCC 431). The ERIC-PCR fingerprinting could become a powerful tool for depicting the genetic diversity among eighteen RNB strains and standard strains. The data based on ERIC fingerprinting pattern could help to determine phylogenetic relationships among these RNB strains and will be helpful for development of diagnostic primer for identification of efficient strains of Chickpea Root Nodulating Bacteria.
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Claims 7
Claim · model-written summary
ERIC fingerprinting clustered chickpea root nodule bacteria and standard strains into four major clusters by phylogenetic relationship.
- Taxon
- chickpea
Cicer arietinume0 - Interactor
- Root nodule bacteriae0symbiont
- Outcome
- four major clusterse1
- Qualifier
- as per their phylogenetic relationshipe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e1“Data analysis of ERIC fingerprinting pattern clustered all RNB strains and standard strains into four major clusters as per their phylogenetic relationship”
Claim · model-written summary
65 per cent of chickpea root nodule bacteria strains were closely related to Mesorhizobium ciceri and Mesorhizobium loti.
- Taxon
- chickpeaCicer arietinume0
- Material
- RNB strainse2
- Interactor
- Root nodule bacteriae0symbiont
- Outcome
- closely related toe2
- Value
- 65 per cente2
- Qualifier
- Mesorhizobium ciceri species and Mesorhizobium lotie2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e2“Majority of RNB strains (65 per cent) were closely related to the genus Mesorhizobium ciceri species and Mesorhizobium loti”
Claim · model-written summary
The remaining 40 per cent of chickpea root nodule bacteria strains showed similarity to Rhizobium leguminosarum (MTCC 99) and Agrobacterium tumefaciens (MTCC 431).
- Taxon
- chickpeaCicer arietinume0
- Material
- RNB strainse3
- Interactor
- Root nodule bacteriae0symbiont
- Outcome
- showed similarity toe3
- Value
- 40 per cente3
- Qualifier
- Rhizobium leguminosarum (MTCC 99) and Agrobacterium tumefaciens (MTCC 431)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e3“remaining 40 per cent RNB strains showed similarity to Rhizobium leguminosarum (MTCC 99) and Agrobacterium tumefaciens (MTCC 431)”
Claim · model-written summary
Strain MTCC 4188 (Mesorhizobium ciceri) produced the highest number of ERIC amplicons among the strains.
- Taxon
- chickpeaCicer arietinume0
- Material
- MTCC 4188e4
- Interactor
- Mesorhizobium ciceriMesorhizobium cicerie4symbiont
- Outcome
- no of ampliconse4
- Direction
- higheste4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e4“The strain MTCC 4188 (Mesorhizobium ciceri) produced highest no of amplicons”
Claim · model-written summary
Strain MTCC 120 (Bradyrhizobium japonicum) produced fewer ERIC amplicons than the other strains.
- Taxon
- chickpeaCicer arietinume0
- Material
- MTCC 120e5
- Interactor
- Bradyrhizobium japonicum
Bradyrhizobium japonicume5symbiont - Outcome
- no of ampliconse5
- Direction
- lesse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e5“strain MTCC 120 (Bradyrhizobium japonicum) showed a less no of amplicons”
Claim · model-written summary
ERIC primers generated a total of 320 amplicons across the chickpea root nodule bacteria and standard strains.
- Taxon
- chickpeaCicer arietinume0
- Outcome
- no of ampliconse6
- Value
- 320e6
- Qualifier
- generated by using ERIC primer paire6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e6“The total of 320 no of amplicons was generated by using ERIC primer pair”
Claim · model-written summary
ERIC-PCR fingerprinting could be a powerful tool for depicting genetic diversity among chickpea root nodule bacteria strains and standard strains.
- Taxon
- chickpeaCicer arietinume0
- Outcome
- genetic diversitye7
- Comparator
- standard strainse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eighteen strains of Root nodule bacteria were collected from the chickpea plant”
e7“The ERIC-PCR fingerprinting could become a powerful tool for depicting the genetic diversity among eighteen RNB strains and standard strains”