Abstract
Pectins comprise a major component of the cell wall and much research has focused on degradation of pectins during ripening and senescence. However, little research has been conducted on pectin synthesis, partly due to a lack of commercial availability of UDP-({sup 14}C)galacturonic acid for use as a substrate in assaying galacturonan synthase. We report on the modification and integration of several procedures to synthesize UDP-({sup 14}C) galacturonic acid from commercially available UDP-({sup 14}C)glucuronic acid. A microsomal pellet containing UDP-D-glucuronate-4-epimerase was extracted from 5-day-old mung bean hypocotyls (Phaseolus aureus) and radish roots (Raphanus sativus L.) by ultracentrifugation at 30,000 rpm for 50 min. The UDP-({sup 14}C)galacturonic acid produced was separated from remaining UDP-({sup 14}C)glucuronic acid and other products by electrophoresis in pyridine:acetate:H{sub 2}O on silica gel. Spots were detected by autoradiography, eluted with 80% ethanol, and purified using anion exchange chromatography.
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Claims 1
Claim · model-written summary
Modified procedures synthesized UDP-(14C) galacturonic acid from commercially available UDP-(14C) glucuronic acid, using a microsomal pellet from mung bean and radish.
- Taxon
- mung bean
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Vigna radiatae2 - Gene
- UDP-D-glucuronate-4-epimerasee2
- Outcome
- UDP-({sup 14}C) galacturonic acide1
- Comparator
- commercially available UDP-({sup 14}C)glucuronic acide1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Schema note from the model: comparator holds the labelled starting substrate used for synthesis, not a control
Verbatim
e1“We report on the modification and integration of several procedures to synthesize UDP-({sup 14}C) galacturonic acid from commercially available UDP-({sup 14}C)glucuronic acid”
e2“A microsomal pellet containing UDP-D-glucuronate-4-epimerase was extracted from 5-day-old mung bean hypocotyls (Phaseolus aureus) and radish roots (Raphanus sativus L.)”