Abstract
The host range of Pseudomonas avenae is wide among monocotyledonous plants, but individual strains can infect only one or a few host species. The resistance response of rice cells to pathogens has been previously shown to be induced by a rice-incompatible strain, N1141, but not by a rice-compatible strain, H8301. To clarify the molecular mechanism of the host specificity in P. avenae, a strain-specific antibody that was raised against N1141 cells and then absorbed with H8301 cells was prepared. When a cell extract of strain N1141 was separated by SDS-polyacrylamide gel electrophoresis and immunostained with the N1141 strain-specific antibody, only a flagellin protein was detected. Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells within 6 h of treatment, whereas the H8301 flagellin did not. The hypersensitive cell death could be blocked by pretreatment with anti-N1141 flagellin antibody. Furthermore, a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene, which is thought to be one of the defense-related genes. These results demonstrated that the resistance response in cultured rice cells is induced by the flagellin existing in the incompatible strain of P. avenae but not in the flagellin of the compatible strain.
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Claims 6
Claim · model-written summary
Only flagellin was detected by the N1141 strain-specific antibody in the N1141 cell extract.
- Taxon
- rice
Oryza sativae1 - Interactor
- Pseudomonas avenaee2pathogen
- Gene
- flagellin proteine0
- Outcome
- only a flagellin protein was detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When a cell extract of strain N1141 was separated by SDS-polyacrylamide gel electrophoresis and immunostained with the N1141 strain-specific antibody, only a flagellin protein was detected.”
e1“resistance response of rice cells”
e2“Pseudomonas avenae”
Claim · model-written summary
Purified N1141 flagellin induced hypersensitive cell death in cultured rice cells within 6 h.
- Taxon
- riceOryza sativae0
- Treatment
- Purified N1141 flagelline0
- Interactor
- Pseudomonas avenaee1pathogen
- Gene
- N1141 flagelline0
- Outcome
- hypersensitive cell deathe0
- Direction
- inducede0
- Qualifier
- within 6 h of treatmente0
- Comparator
- H8301 flagelline0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells within 6 h of treatment, whereas the H8301 flagellin did not.”
e1“Pseudomonas avenae”
Claim · model-written summary
H8301 flagellin did not induce hypersensitive cell death in cultured rice cells.
- Taxon
- riceOryza sativae0
- Treatment
- H8301 flagelline0
- Interactor
- Pseudomonas avenaee1pathogen
- Outcome
- hypersensitive cell deathe0
- Direction
- did note0
- Comparator
- Purified N1141 flagelline0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells within 6 h of treatment, whereas the H8301 flagellin did not.”
e1“Pseudomonas avenae”
Claim · model-written summary
Pretreatment with anti-N1141 flagellin antibody blocked hypersensitive cell death in cultured rice cells.
- Taxon
- riceOryza sativae1
- Treatment
- pretreatment with anti-N1141 flagellin antibodye0
- Interactor
- Pseudomonas avenaee2pathogen
- Outcome
- hypersensitive cell deathe0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypersensitive cell death could be blocked by pretreatment with anti-N1141 flagellin antibody.”
e1“resistance response of rice cells”
e2“Pseudomonas avenae”
Claim · model-written summary
Flagellin-deficient N1141 lost its ability to induce hypersensitive cell death in cultured rice cells.
- Taxon
- riceOryza sativae1
- Treatment
- flagellin-deficient N1141 straine0
- Interactor
- Pseudomonas avenaee2pathogen
- Gene
- flagelline0
- Outcome
- induction ability of hypersensitive cell deathe0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene”
e1“resistance response of rice cells”
e2“Pseudomonas avenae”
Claim · model-written summary
Flagellin-deficient N1141 lost its ability to induce EL2 expression in cultured rice cells.
- Taxon
- riceOryza sativae1
- Treatment
- flagellin-deficient N1141 straine0
- Interactor
- Pseudomonas avenaee2pathogen
- Gene
- EL2 genee0
- Outcome
- expression ability of the EL2 genee0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene”
e1“resistance response of rice cells”
e2“Pseudomonas avenae”