Abstract
Tissue damage creates numerous problems for the microscopic examination of resting peanut (Arachis hypogaea L. cv . Florunner) seed physical structure. Th1s paper presents a method to deal with specific problems encountered in light micros copy (LM) and scanning electron microscopy (SEM) of drought - induced damage of peanut tissue . Major findings include: (1) improved SEM imaging through reduction of charging by increasing coating thickness from 25 nm to 30 nm; (2) improved stain affinity for LM tissue preparations by decreasing osmium tetrox ide fixation time from 2 hours to 1 hour; and (3) im proved tissue fixation for LM and SE M by application of a modified Karnovsky fixative (glutaraldehyde / formaldehyde combination) which proved more suc cessful than glutaraldehyde alone for tissue preservation.
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Claims 3
Claim · model-written summary
Increasing the SEM coating thickness from 25 nm to 30 nm improved SEM imaging of peanut seed tissue by reducing charging.
- Taxon
- peanut
Arachis hypogaeae0 - Material
- Florunnere0
- Organ / stage
- seede0
- Treatment
- increasing coating thickness from 25 nm to 30 nme1
- Outcome
- SEM imaginge1
- Value
- 30 nme1
- Comparator
- 25 nme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tissue damage creates numerous problems for the microscopic examination of resting peanut (Arachis hypogaea L. cv . Florunner) seed physical structure”
e1“improved SEM imaging through reduction of charging by increasing coating thickness from 25 nm to 30 nm”
Claim · model-written summary
Reducing osmium fixation time from 2 hours to 1 hour improved stain affinity for light microscopy of peanut seed tissue.
- Taxon
- peanutArachis hypogaeae0
- Outcome
- stain affinitye3
- Value
- 1 houre2
- Comparator
- 2 hourse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tissue damage creates numerous problems for the microscopic examination of resting peanut (Arachis hypogaea L. cv . Florunner) seed physical structure”
e2“from 2 hours to 1 hour”
e3“improved stain affinity for LM tissue preparations”
Claim · model-written summary
A modified Karnovsky fixative (glutaraldehyde / formaldehyde combination) was more successful than glutaraldehyde alone for tissue preservation of peanut seed tissue.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- a modified Karnovsky fixative (glutaraldehyde / formaldehyde combination)e4
- Outcome
- tissue preservatione5
- Comparator
- glutaraldehyde alonee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tissue damage creates numerous problems for the microscopic examination of resting peanut (Arachis hypogaea L. cv . Florunner) seed physical structure”
e4“a modified Karnovsky fixative (glutaraldehyde / formaldehyde combination)”
e5“than glutaraldehyde alone for tissue preservation”