Abstract
Currently, most organelle isolation procedures rely on physical parameters and centrifugation for separation. Here, we report the rapid and gentle isolation of a variety of organelles by immunolabeling whole cell lysates with organelle-specific antibodies and streptavidin magnetic particles followed by separation in a magnetic field. Using magnetic immunoabsorption, we have been able to specifically label mouse metaphase chromosomes and a variety of plant organelles, including: amyloplasts, choroplasts and nuclei from whole cell lysates of various plant tissues. We find that the distinct magnetic properties, surface characteristics and mean diameter-size ranges of different particle preparations significantly influence their specific utility for organelle isolations. By using an internal-field magnetic separation device, we have developed a method for quantitative recovery of labeled organelles in microarrays and tested a variety of antibodies to chloroplast outer envelope proteins for their ability to immune-isolate chloroplasts.
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Claims 3
Claim · model-written summary
Magnetic immunoabsorption specifically labeled amyloplasts, chloroplasts and nuclei from plant tissues.
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Verbatim
e0“Using magnetic immunoabsorption, we have been able to specifically label mouse metaphase chromosomes and a variety of plant organelles, including: amyloplasts, choroplasts and nuclei from whole cell lysates of various plant tissues.”
Claim · model-written summary
Magnetic particle properties significantly influenced utility for organelle isolation.
Not reportedDirection
Verbatim
e0“the distinct magnetic properties, surface characteristics and mean diameter-size ranges of different particle preparations significantly influence their specific utility for organelle isolations”
e7“a variety of plant organelles”
Claim · model-written summary
An internal-field magnetic separator enabled quantitative recovery of labeled organelles in microarrays.
Not reportedComparatorDirectionValue
Verbatim
e0“By using an internal-field magnetic separation device, we have developed a method for quantitative recovery of labeled organelles in microarrays”
e7“a variety of plant organelles”