Paper · 1999 · Agritrop (Cirad)

The interspecific genome structure of cultivated banana, Musa spp. revealed by flow cytometry and genomic DNA in situ hybridisation

Angélique D’Hont; A. Paget Goy; Christophe Jenny; et al.

7
claims
7
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

The genomes of four different wild Musa species are involved in cultivated bananas : the A, B, S and T genomes related to M. acuminata, M. balbisiana, M. schizocarpa and the Australimusa species, respectively. Most cultivated clones are triploid or diploid. They have been classified into genomic groups according to chromosome numbers and morphological traits. The main groups are AA, AAA, AAB, ABB, AS or AT. Flow cytometry reveals that the sizes of A, B, S and T genomes are significantly different. M. balbisiana has the smallest genome (1.03 pg/2C, 2n=2x=22), followed by M. acuminata (1.11 pg/2C, 2n=2x=22) and M. schizocarpa (1.18 pg/2C, 2n=2x =22). The highest value was obtained for the Australimusa species M. textilis (1.27 pg/2C, 2n=2x=20). Genome size can thus be used to determine the species involved in interspecific diploid clones. Genomic in situ hybridization has been developed to characterize the specific origin of the chromosomes in interspecific clones. This technique enables us to differentiate the chromosomes from the genomes A, B, S and T and is being used to determine the exact genome structure of cultivated clones and synthetic hybrids. (Texte intégral)

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

A, B, S and T genomes differed significantly in size.

Outcome
sizes of A, B, S and T genomese0
Direction
differente0

Not reportedTaxon

Verbatim

e0

“Flow cytometry reveals that the sizes of A, B, S and T genomes are significantly different.”

✓ located verbatim in the source · abstract, characters 411–502 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

M. balbisiana had the smallest genome at 1.03 pg/2C.

Taxon
M. balbisianaMusa balbisianae0
Outcome
genomee0
Direction
smalleste0
Value
1.03 pg/2Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Musa balbisiana
Musa balbisiana · TaxonXufanc · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“M. balbisiana has the smallest genome (1.03 pg/2C, 2n=2x=22)”

✓ located verbatim in the source · abstract, characters 503–563 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

M. acuminata genome size was 1.11 pg/2C.

Taxon
M. acuminataMusa acuminatae0
Outcome
M. acuminatae0
Value
1.11 pg/2Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Musa acuminata
Musa acuminata · TaxonJorgemach7 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“M. acuminata (1.11 pg/2C, 2n=2x=22)”

✓ located verbatim in the source · abstract, characters 577–612 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

M. textilis had the highest genome size at 1.27 pg/2C.

Taxon
M. textilisMusa textilise0
Outcome
highest valuee0
Direction
higheste0
Value
1.27 pg/2Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Musa textilis
Musa textilis · TaxonMarvinBikolano · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest value was obtained for the Australimusa species M. textilis (1.27 pg/2C, 2n=2x=20).”

✓ located verbatim in the source · abstract, characters 657–752 · show in text ↑
Method / resourceauthor interpretationoriginal resultmeasured

Claim · model-written summary

Genome size distinguished species contributions to interspecific diploid clones.

Outcome
determine the species involved in interspecific diploid clonese0
Comparator
interspecific diploid clonese0

Not reportedTaxon

Verbatim

e0

“Genome size can thus be used to determine the species involved in interspecific diploid clones.”

✓ located verbatim in the source · abstract, characters 753–848 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Genomic in situ hybridization differentiated chromosomes from the A, B, S and T genomes.

Outcome
differentiate the chromosomes from the genomes A, B, S and Te0
Comparator
cultivated clones and synthetic hybridse0

Not reportedTaxon

Verbatim

e0

“This technique enables us to differentiate the chromosomes from the genomes A, B, S and T and is being used to determine the exact genome structure of cultivated clones and synthetic hybrids.”

✓ located verbatim in the source · abstract, characters 978–1169 · show in text ↑