Paper · 1979 · Journal of AOAC INTERNATIONAL

Production of Aflatoxin in Cocoa Beans

Lawrence M Lenovich; W Jeffrey Hurst

8
claims
14
verbatim quotes
10
citations
3
research areas
✓
Open access

Abstract

Aflatoxin was produced in both non-autoclaved and autoclaved Ivory Coast cocoa beans inoculated with Aspergillus parasiticus NRRL 2999 under optimum laboratory growth conditions. Total aflatoxin levels ranged from 213 to 5597 ng/g substrate. Aflatoxin was quantitated by using high pressure liquid chromatography (HPLC). Raw, non-autoclaved cocoa beans, also inoculated with aspergilli, produced 6359 ng aflatoxin/g substrate. Variation in aflatoxin production between bean varieties was observed. Total aflatoxin levels of 10,446 and 23,076 ng/g substrate were obtained on Ivory Coast beans inoculated with A. parasiticus NRRL 2999 and NRRL 3240, respectively. Aflatoxin production on Trinidad and Malaysian beans was 28 and 65 ng aflatoxin/g substrate. These data support previously reported low level natural aflatoxin contamination in cocoa.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Aflatoxin formed in inoculated Ivory Coast cocoa beans under laboratory conditions.

Taxon
cocoa beanse0
Interactor
Aspergillus parasiticus NRRL 2999Aspergillus parasiticuse0pathogen
Outcome
Aflatoxine0
Setting
laboratory growth conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Aspergillus parasiticus
Aspergillus parasiticus · InteractorMedmyco · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Aflatoxin was produced in both non-autoclaved and autoclaved Ivory Coast cocoa beans inoculated with Aspergillus parasiticus NRRL 2999 under optimum laboratory growth conditions.”

✓ located verbatim in the source · abstract, characters 0–178 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Total aflatoxin in inoculated beans ranged from 213 to 5597 ng/g substrate.

Taxon
Cocoa Beanst0
Outcome
Total aflatoxin levelse0
Value
213 to 5597 ng/g substratee0

Verbatim

e0

“Total aflatoxin levels ranged from 213 to 5597 ng/g substrate.”

✓ located verbatim in the source · abstract, characters 179–241 · show in text ↑
t0

“Cocoa Beans”

✓ located verbatim in the source · title, characters 27–38 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Raw non-autoclaved inoculated cocoa beans produced 6359 ng aflatoxin/g substrate.

Taxon
cocoa beanse0
Interactor
aspergillie0pathogen
Outcome
aflatoxine0
Value
6359 ng aflatoxin/g substratee0

Verbatim

e0

“Raw, non-autoclaved cocoa beans, also inoculated with aspergilli, produced 6359 ng aflatoxin/g substrate.”

✓ located verbatim in the source · abstract, characters 321–426 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Aflatoxin production varied between bean varieties.

Taxon
Cocoa Beanst0
Material
bean varietiese0
Outcome
aflatoxin productione0

Not reportedDirection

Verbatim

e0

“Variation in aflatoxin production between bean varieties was observed.”

✓ located verbatim in the source · abstract, characters 427–497 · show in text ↑
t0

“Cocoa Beans”

✓ located verbatim in the source · title, characters 27–38 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ivory Coast beans inoculated with NRRL 2999 had 10,446 ng/g substrate total aflatoxin.

Taxon
Cocoa Beanst0
Material
Ivory Coast beanse0
Interactor
A. parasiticus NRRL 2999Aspergillus parasiticuse0pathogen
Outcome
Total aflatoxin levelse0
Value
10,446e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Aspergillus parasiticus
Aspergillus parasiticus · InteractorMedmyco · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Total aflatoxin levels of 10,446 and 23,076 ng/g substrate were obtained on Ivory Coast beans inoculated with A. parasiticus NRRL 2999 and NRRL 3240, respectively.”

✓ located verbatim in the source · abstract, characters 498–661 · show in text ↑
t0

“Cocoa Beans”

✓ located verbatim in the source · title, characters 27–38 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ivory Coast beans inoculated with NRRL 3240 had 23,076 ng/g substrate total aflatoxin.

Taxon
Cocoa Beanst0
Material
Ivory Coast beanse0
Interactor
NRRL 3240e0pathogen
Outcome
Total aflatoxin levelse0
Value
23,076 ng/g substratee0

Verbatim

e0

“Total aflatoxin levels of 10,446 and 23,076 ng/g substrate were obtained on Ivory Coast beans inoculated with A. parasiticus NRRL 2999 and NRRL 3240, respectively.”

✓ located verbatim in the source · abstract, characters 498–661 · show in text ↑
t0

“Cocoa Beans”

✓ located verbatim in the source · title, characters 27–38 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Trinidad beans produced 28 ng aflatoxin/g substrate.

Taxon
Cocoa Beanst0
Material
Trinidade0
Outcome
Aflatoxin productione0
Value
28e0

Verbatim

e0

“Aflatoxin production on Trinidad and Malaysian beans was 28 and 65 ng aflatoxin/g substrate.”

✓ located verbatim in the source · abstract, characters 662–754 · show in text ↑
t0

“Cocoa Beans”

✓ located verbatim in the source · title, characters 27–38 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Malaysian beans produced 65 ng aflatoxin/g substrate.

Taxon
Cocoa Beanst0
Material
Malaysiane0
Outcome
Aflatoxin productione0
Value
65 ng aflatoxin/g substratee0

Verbatim

e0

“Aflatoxin production on Trinidad and Malaysian beans was 28 and 65 ng aflatoxin/g substrate.”

✓ located verbatim in the source · abstract, characters 662–754 · show in text ↑
t0

“Cocoa Beans”

✓ located verbatim in the source · title, characters 27–38 · show in text ↑