Abstract
Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed by chromatography on methylated albumin kieselguhr (MAK) columns. Treatment with actinomycin D reduced the synthesis of ribosomal ribonucleic acid (RNA) to low levels and allowed the detection of the three components of BMV-RNA in vivo. The kinetic study on (32)P incorporation into these BMV-RNA components suggested that a single cleavage occurred in some of the intact RNA shortly after completion of its synthesis, giving rise to the small and medium components. Chromatographic analyses also revealed a double-stranded, ribonuclease-resistant RNA which has been purified by differential extraction, sucrose-density gradient centrifugation, and MAK column chromatography. This RNA sediments at approximately 14S, is alkali-labile, and has a sharp thermal transition with a T(m) of 96 C in 0.1 x standard saline citrate buffer, as determined by susceptibility to ribonuclease. The RNA is absent in uninfected barley plants.
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Claims 8
Claim · model-written summary
Actinomycin D treatment reduced ribosomal RNA synthesis to low levels in barley plants infected with bromegrass mosaic virus.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- actinomycin De1
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- synthesis of ribosomal ribonucleic acid (RNA)e1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“Treatment with actinomycin D reduced the synthesis of ribosomal ribonucleic acid (RNA) to low levels”
Claim · model-written summary
With actinomycin D, the three components of BMV-RNA could be detected in vivo.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- detection of the three components of BMV-RNAe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“allowed the detection of the three components of BMV-RNA in vivo”
Claim · model-written summary
Kinetics of 32P incorporation suggested a single cleavage in some intact BMV RNA shortly after synthesis, giving the small and medium components.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- small and medium componentse1
- Qualifier
- single cleavagee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“The kinetic study on (32)P incorporation into these BMV-RNA components suggested that a single cleavage occurred in some of the intact RNA shortly after completion of its synthesis, giving rise to the small and medium components”
Claim · model-written summary
A double-stranded, ribonuclease-resistant RNA was revealed by chromatography in infected barley plants.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- double-stranded, ribonuclease-resistant RNAe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“Chromatographic analyses also revealed a double-stranded, ribonuclease-resistant RNA”
Claim · model-written summary
The double-stranded RNA sediments at approximately 14S.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- sedimentse1
- Value
- 14Se1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“This RNA sediments at approximately 14S”
Claim · model-written summary
The double-stranded RNA is alkali-labile.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- alkali-labilee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“is alkali-labile”
Claim · model-written summary
The double-stranded RNA has a sharp thermal transition with a Tm of 96 C in 0.1 x standard saline citrate buffer.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- sharp thermal transitione1
- Value
- 96 Ce1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“has a sharp thermal transition with a T(m) of 96 C in 0.1 x standard saline citrate buffer”
Claim · model-written summary
The double-stranded RNA is absent in uninfected barley plants.
- Taxon
- barleyHordeum vulgaree0
- Interactor
- bromegrass mosaic viruse0pathogen
- Outcome
- absente1
- Comparator
- uninfected barley plantse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of (32)P into nucleic acids in barley plants infected with bromegrass mosaic virus (BMV) was analyzed”
e1“The RNA is absent in uninfected barley plants”