Abstract
Intensification of biofuel resources is urgently needed considering the decrease in availability of world’s fossil fuel. Palmyra palm (Borassus flabellifer) is highly potential to be developed as bioethanol source regarding the high sucrose content in its nira. It was observed that nira produced in dry season is sweeter than that in rainy season, which presumed to be influenced by a difference in expression level of sucrose-related genes during the two seasons. Studies on Sucrose Synthase (SUS) gene of palmyra are therefore required prior to study the gene expression. Palmyra SUS gene sequence is currently unavailable in GenBank, thereby pair of primers was designed from highly conserved region of SUS proteins among monocots. A 1866 bp partial cDNA fragment of SUS putative gene has been succesfully isolated from RNA of the young leaves of B. flabellifer. BLASTn and BLASTp aligments showed that either BfSUS cDNA or BfSUS polypeptide has high similarity with SUS cDNA and proteins from diverse plant species, with the highest similarity shown by Tulipa gesneriana. The phylogenetic tree showed that SUS protein sequences of monocot species were distinctively grouped and splitted from those of dicot species. The BfSUS was clustered in monocot group, although not specifically grouped with particular monocot species. Nevertheless, B. flabellifer showed nearest genetic distance with Tulipa gesneriana and Oncidium cv.’Goldiana’. Characterization of BfSUS polypeptide using Geneious 4.6.2 indicated the presence of sucrose synthase (SUS) and glycosyl transferase (GT) domains, four putative UDP-glucose binding pockets within the GT domain, and a calcium-dependent Ser/Thr protein kinase binding site within the SUS domain. These domains and motifs are highly conserved in SUS proteins across plant species, hence confirming that the cDNA fragment obtained in this study is the putative gene of sucrose synthase of B. flabellifer. Keywords: Borassus flabellifer, nira, sucrose, sucrose synthase, SUS domain, GT domain, Â
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Claims 9
Claim · model-written summary
A 1866 bp partial putative sucrose synthase cDNA was isolated from young B. flabellifer leaves.
- Taxon
- B. flabellifer
Borassus flabellifere0 - Organ / stage
- young leavese0
- Gene
- SUS putative genee0
- Outcome
- partial cDNA fragmente0
- Value
- 1866 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 1866 bp partial cDNA fragment of SUS putative gene has been succesfully isolated from RNA of the young leaves of B. flabellifer”
Claim · model-written summary
BfSUS cDNA and polypeptide showed high similarity to other plant SUS sequences, highest with Tulipa gesneriana.
Verbatim
e0“either BfSUS cDNA or BfSUS polypeptide has high similarity with SUS cDNA and proteins from diverse plant species, with the highest similarity shown by Tulipa gesneriana”
t0“PALMYRA PALM”
Claim · model-written summary
Monocot SUS proteins clustered separately from dicot SUS proteins.
Verbatim
e0“SUS protein sequences of monocot species were distinctively grouped and splitted from those of dicot species”
t0“PALMYRA PALM”
Claim · model-written summary
BfSUS clustered among monocots without a particular species grouping.
Verbatim
e0“The BfSUS was clustered in monocot group, although not specifically grouped with particular monocot species”
t0“PALMYRA PALM”
Claim · model-written summary
B. flabellifer was genetically nearest Tulipa gesneriana and Oncidium cv. Goldiana.
- Taxon
- B. flabelliferBorassus flabellifere0
- Outcome
- nearest genetic distancee0
- Comparator
- Tulipa gesneriana and Oncidium cv.’Goldiana’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. flabellifer showed nearest genetic distance with Tulipa gesneriana and Oncidium cv.’Goldiana’”
Claim · model-written summary
BfSUS polypeptide contained SUS and GT domains.
Verbatim
e0“presence of sucrose synthase (SUS) and glycosyl transferase (GT) domains”
t0“PALMYRA PALM”
e1“The BfSUS was clustered in monocot group”
Claim · model-written summary
BfSUS contained four putative UDP-glucose binding pockets in the GT domain.
Verbatim
e0“four putative UDP-glucose binding pockets within the GT domain”
t0“PALMYRA PALM”
e1“The BfSUS was clustered in monocot group”
Claim · model-written summary
BfSUS contained a calcium-dependent Ser/Thr protein kinase binding site in the SUS domain.
Verbatim
e0“a calcium-dependent Ser/Thr protein kinase binding site within the SUS domain”
t0“PALMYRA PALM”
e1“The BfSUS was clustered in monocot group”
Claim · model-written summary
The conserved domains and motifs supported identification of the fragment as putative palmyra sucrose synthase.
- Taxon
- B. flabelliferBorassus flabellifere0
- Gene
- sucrose synthasee0
- Outcome
- putative gene of sucrose synthasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These domains and motifs are highly conserved in SUS proteins across plant species, hence confirming that the cDNA fragment obtained in this study is the putative gene of sucrose synthase of B. flabellifer”