Abstract
Intact soil-core microcosms were studied to determine their applicability for evaluating the transport, survival, and potential ecosystem effects of genetically engineered microorganisms before they are released into the environment. Soil-core microcosms were planted with wheat and maize seeds and inoculated with Azospirillum lipoferum SpBr17 and SpRG20a Tn5 mutants, respectively. Microcosm leachate, rhizosphere soil, plant endorhizosphere, insects, and xylem exudate were sampled for A. lipoferum Tn5 mutant populations. A. lipoferum Tn5 populations, determined by most-probable-number technique-DNA hybridization, varied from below detection to 10 g of dry root in the rhizosphere, with smaller populations detected in the endorhizosphere. Intact soil-core microcosms were found to maintain some of the complexities of the natural ecosystem and should be particularly useful for initial evaluations of the fate of plant-associated genetically engineered bacteria.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 2
Claim · model-written summary
Azospirillum lipoferum mutant populations ranged from below detection to the reported upper rhizosphere level, with smaller endorhizosphere populations.
- Taxon
- wheat
Triticum aestivume1 - Organ / stage
- rhizospheree0
- Interactor
- A. lipoferum Tn5Azospirillum lipoferume0symbiont
- Outcome
- A. lipoferum Tn5 populationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. lipoferum Tn5 populations, determined by most-probable-number technique-DNA hybridization, varied from below detection to 10 g of dry root in the rhizosphere, with smaller populations detected in the endorhizosphere”
e1“wheat and maize seeds”
Claim · model-written summary
Planted soil-core microcosms retained some natural ecosystem complexity and were judged useful for initial evaluations of engineered plant-associated bacteria.
Verbatim
e0“Intact soil-core microcosms were found to maintain some of the complexities of the natural ecosystem and should be particularly useful for initial evaluations of the fate of plant-associated genetically engineered bacteria”