Abstract
The sensing of stress signals and their transduction into appropriate responses are crucial for the adaptation, survival and infection of phytopathogenic fungi and oomycetes. Amongst evolutionarily conserved pathways, mitogen-activated protein kinase (MAPK) cascades function as key signal transducers that use phosphorylation to convey information. In this study, we identified a gene, designated PsMPK7, one of 14 predicted genes encoding MAPKs in Phytophthora sojae. PsMPK7 was highly transcribed in each tested stage, but was up-regulated in the zoospore, cyst and cyst germination stages. Silencing of PsMPK7 affected the growth of germinated cysts, oospore production and the pathogenicity of soybean. PsMPK7 transcription was induced by stresses from sorbitol, NaCl and hydrogen peroxide. Transformants in which PsMPK7 expression was silenced (PsMPK7-silenced) were significantly more sensitive to osmotic and oxidative stress. Aniline blue and diaminobenzidine staining revealed that the silenced lines did not suppress the host reactive oxygen species (ROS) burst, indicating that either the inoculated plants activated stronger defence responses to the transformants and/or the PsMPK7-silenced transformants failed to overcome plant defences. In addition, extracellular secretion of laccase decreased in the silenced lines. Overall, our results indicate that the PsMPK7 gene encodes a stress-associated MAPK in P. sojae that is important not only for responses to various stresses, but also for ROS detoxification, cyst germination, sexual oospore production and infection of soybean.
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Claims 2
Claim · model-written summary
PsMPK7 silenced Phytophthora lines failed to suppress the soybean host ROS burst.
- Taxon
- soybean
Glycine maxt0 - Material
- silenced linese0
- Interactor
- P hytophthora sojaet1pathogen
- Gene
- PsMPK7t1
- Outcome
- host reactive oxygen species (ROS) burste0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aniline blue and diaminobenzidine staining revealed that the silenced lines did not suppress the host reactive oxygen species (ROS) burst”
t0“soybean”
t1“PsMPK7 , a stress‐associated mitogen‐activated protein kinase ( MAPK ) in P hytophthora sojae”
Claim · model-written summary
Silencing PsMPK7 affected Phytophthora pathogenicity on soybean.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Silencing of PsMPK7e0
- Gene
- PsMPK7e0
- Outcome
- pathogenicity of soybeane0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of PsMPK7 affected the growth of germinated cysts, oospore production and the pathogenicity of soybean.”
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