Abstract
Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea. Biological properties of the isolate named PVY-ToBR1 was characterized using various host plants with another isolate PVY-ToJC37. The isolated PVY-ToBR1 induced systemic vein necrosis symptoms on a PVY-resistant tobacco cultivar (VAM) harboring potyvirus resistant va gene, though tissue printing showed the systemic movement of virus was slightly delayed. By contrast, the isolate PVY-ToJC37 failed to infect VAM plants and the virus was not detected on inoculated leaf and systemic leaves in VAM plants. Similarly, the isolated PVY-ToBR1 induced distinctly systemic vein necrosis symptoms on PVY-resistant tobacco cultivars (V.SCR, PBD6, TN86, TN90, Virgin A Mutant, NC744, and Wislica) that have the recessive potyvirus resistance gene va, but PVY-ToJC37 did not infect systemically infect these tobacco cultivars, suggesting that PVY-ToBR1 is a novel resistance-breaking isolate in tobacco. The coat protein (CP) genes of PVY-ToBR1 and PVY-ToJC37 were amplified using RT-PCR assays with specific primers for PVY isolates and nucleotide sequences of the CP genes were determined. The isolate PVY-ToBR1 showed 88.4% - 99.4% and 86.6% - 99.4% CP identities to the 46 different PVY isolates at the nucleotide and amino acid, respectively. Phylogenetic relationship from CP comparisons showed that PVY-ToBR1 isolate clustered with PVYNTN isolates and PVY-ToBR1 isolate more closely related to the isolates from European than from North American PVY NTN.
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Claims 8
Claim · model-written summary
PVY-ToBR1 induced systemic vein necrosis symptoms on the PVY-resistant tobacco cultivar VAM.
- Taxon
- tobacco
Nicotiana tabacume1 - Material
- VAMe2
- Interactor
- PVY-ToBR1e2pathogen
- Outcome
- systemic vein necrosis symptomse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e2“The isolated PVY-ToBR1 induced systemic vein necrosis symptoms on a PVY-resistant tobacco cultivar (VAM) harboring potyvirus resistant va gene, though tissue printing showed the systemic movement of virus was slightly delayed”
Claim · model-written summary
Systemic movement of PVY-ToBR1 in VAM plants was slightly delayed.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- VAMe2
- Interactor
- PVY-ToBR1e2pathogen
- Outcome
- systemic movement of viruse2
- Direction
- slightly delayede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e2“The isolated PVY-ToBR1 induced systemic vein necrosis symptoms on a PVY-resistant tobacco cultivar (VAM) harboring potyvirus resistant va gene, though tissue printing showed the systemic movement of virus was slightly delayed”
Claim · model-written summary
PVY-ToJC37 failed to infect VAM plants, and the virus was not detected in inoculated or systemic leaves.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- VAMe3
- Interactor
- PVY-ToJC37e3pathogen
- Outcome
- the virus was not detected on inoculated leaf and systemic leavese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e3“By contrast, the isolate PVY-ToJC37 failed to infect VAM plants and the virus was not detected on inoculated leaf and systemic leaves in VAM plants”
Claim · model-written summary
PVY-ToBR1 induced systemic vein necrosis symptoms on PVY-resistant tobacco cultivars carrying the recessive va gene.
- Taxon
- tobaccoNicotiana tabacume4
- Material
- V.SCR, PBD6, TN86, TN90, Virgin A Mutant, NC744, and Wislicae4
- Interactor
- PVY-ToBR1e4pathogen
- Gene
- vae4
- Outcome
- systemic vein necrosis symptomse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e4“Similarly, the isolated PVY-ToBR1 induced distinctly systemic vein necrosis symptoms on PVY-resistant tobacco cultivars (V.SCR, PBD6, TN86, TN90, Virgin A Mutant, NC744, and Wislica) that have the recessive potyvirus resistance gene va”
Claim · model-written summary
The CP sequence of PVY-ToBR1 showed 88.4% - 99.4% nucleotide identity to 46 PVY isolates.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVY-ToBR1e6pathogen
- Outcome
- CP identitiese6
- Value
- 88.4% - 99.4%e6
- Qualifier
- at the nucleotidee6
- Comparator
- 46 different PVY isolatese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e6“The isolate PVY-ToBR1 showed 88.4% - 99.4% and 86.6% - 99.4% CP identities to the 46 different PVY isolates at the nucleotide and amino acid, respectively”
Claim · model-written summary
The CP sequence of PVY-ToBR1 showed 86.6% - 99.4% amino acid identity to 46 PVY isolates.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVY-ToBR1e6pathogen
- Outcome
- CP identitiese6
- Value
- 86.6% - 99.4%e6
- Qualifier
- amino acide6
- Comparator
- 46 different PVY isolatese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e6“The isolate PVY-ToBR1 showed 88.4% - 99.4% and 86.6% - 99.4% CP identities to the 46 different PVY isolates at the nucleotide and amino acid, respectively”
Claim · model-written summary
PVY-ToBR1 clustered with PVYNTN isolates in the phylogenetic analysis of CP sequences.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVY-ToBR1e7pathogen
- Outcome
- clustered with PVYNTN isolatese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e7“Phylogenetic relationship from CP comparisons showed that PVY-ToBR1 isolate clustered with PVYNTN isolates”
Claim · model-written summary
PVY-ToBR1 is more closely related to European than to North American PVY NTN isolates.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVY-ToBR1e7pathogen
- Outcome
- more closely related to the isolates from Europeane7
- Direction
- more closely relatede7
- Comparator
- from North American PVY NTNe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea”
e7“PVY-ToBR1 isolate more closely related to the isolates from European than from North American PVY NTN”