Abstract
A partial ( c. 3 kb) nucleotide sequence of the genome of Raspberry vein chlorosis virus (RVCV) has been obtained, confirming that it is a plant rhabdovirus. Sequence data were obtained using two approaches, conventional reverse transcriptase polymerase chain reaction (RT‐PCR) of single‐strand RNA using degenerate primers designed from rhabdovirus L gene sequences, and mass (454) sequencing of randomly‐amplified double‐stranded RNA. Amplification using the degenerate primers of RNA extracted from virus‐infected raspberry plants was unsuccessful, whereas amplification of rhabdovirus sequences from RNA extracts of the RVCV vector aphid, Aphis idaei , was successful. From these sequences, RVCV‐specific primers were designed which could be used to direct amplification of fragments of the viral genome both from infected aphids and from infected raspberry plants. Phylogenetic analysis of available rhabdovirus sequences shows that RVCV is most closely related to Strawberry crinkle virus .
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Claims 5
Claim · model-written summary
A partial sequence of the RVCV genome confirms that it is a plant rhabdovirus.
- Taxon
- raspberryt0
- Interactor
- Raspberry vein chlorosis virus
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Raspberry vein chlorosis virust0pathogen - Outcome
- partial ( c. 3 kb) nucleotide sequence of the genomee0
- Qualifier
- confirming that it is a plant rhabdoviruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A partial ( c. 3 kb) nucleotide sequence of the genome of Raspberry vein chlorosis virus (RVCV) has been obtained, confirming that it is a plant rhabdovirus”
t0“Raspberry vein chlorosis virus”
Claim · model-written summary
Amplification with degenerate primers from RNA of virus-infected raspberry plants was unsuccessful.
Verbatim
e1“Amplification using the degenerate primers of RNA extracted from virus‐infected raspberry plants was unsuccessful, whereas amplification of rhabdovirus sequences from RNA extracts of the RVCV vector aphid, Aphis idaei , was successful”
Claim · model-written summary
Amplification of rhabdovirus sequences from RNA of the RVCV vector aphid was successful.
- Taxon
- raspberrye1
- Interactor
- Aphis idaei
Aphis idaeie1pest - Outcome
- amplification of rhabdovirus sequencese1
- Comparator
- RNA extracted from virus‐infected raspberry plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Amplification using the degenerate primers of RNA extracted from virus‐infected raspberry plants was unsuccessful, whereas amplification of rhabdovirus sequences from RNA extracts of the RVCV vector aphid, Aphis idaei , was successful”
Claim · model-written summary
RVCV-specific primers amplified fragments of the viral genome from infected aphids and from infected raspberry plants.
Verbatim
e2“RVCV‐specific primers were designed which could be used to direct amplification of fragments of the viral genome both from infected aphids and from infected raspberry plants”
Claim · model-written summary
RVCV is most closely related to Strawberry crinkle virus among the available rhabdovirus sequences.
Verbatim
e3“Phylogenetic analysis of available rhabdovirus sequences shows that RVCV is most closely related to Strawberry crinkle virus”
t0“Raspberry vein chlorosis virus”