Abstract
The relative genetic similarities of 200 isolates of Rhizobium leguminosarum bv. trifolii recovered from an Oregon soil were determined at 13 enzyme loci by multilocus enzyme electrophoresis (MLEE). These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species. The MLEE-derived levels of relatedness among isolates of R. leguminosarum bv. trifolii were found to be in good agreement with the levels of relatedness established by using repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the PCR technique and with levels of relatedness from previously published DNA reassociation studies. BIOLOG substrate utilization patterns showed that isolates within an electrophoretic type (ET) were phenotypically more similar to each other than to isolates of other ETs. The soil isolates were represented by 53 ETs which could be clustered into seven groups (groups B, E, G, H1, H2, I, and J). Evidence for multilocus structure within the population was obtained, and group B was identified as the primary creator of the disequilibrium. Of 75 isolates belonging to the nodule-dominant serotype AS6 complex, 72 were found in group B. Isolates WS2-01 and WS2-02 representing nodule-dominant serotypes recovered from subclover grown at another Oregon site were also found in group B. Isolates representing the most numerous ETs in group B (ETs 2 and 3) were either suboptimally effective or completely ineffective at fixing nitrogen on six different clover species. Another four groups of isolates (groups A, C, D, and F) were identified when 32 strains of diverse origins were analyzed by MLEE and incorporated into the cluster analysis. Group A was most dissimilar in comparisons with other groups and contained strain USDA 2124 (T24), which produces trifolitoxin and has unique symbiotic characteristics.
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Claims 9
Claim · model-written summary
The soil isolates were represented by 53 electrophoretic types clustered into seven groups.
- Interactor
- Rhizobium leguminosarum bv. trifoliiRhizobium leguminosarum bv. trifoliit0symbiont
- Outcome
- clustered into seven groupse2
- Value
- 53 ETse2
TrifoliumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rhizobium leguminosarum bv. trifolii”
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e2“The soil isolates were represented by 53 ETs which could be clustered into seven groups”
Claim · model-written summary
MLEE levels of relatedness were in good agreement with relatedness from repetitive sequence PCR and previously published DNA reassociation studies.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rhizobium leguminosarum bv. trifolii”
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e3“The MLEE-derived levels of relatedness among isolates of R. leguminosarum bv. trifolii were found to be in good agreement with the levels of relatedness established by using repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the PCR technique and with levels of relatedness from previously published DNA reassociation studies”
Claim · model-written summary
Isolates within an electrophoretic type were phenotypically more similar to each other than to isolates of other types, by BIOLOG profiles.
- Interactor
- Rhizobium leguminosarum bv. trifoliiRhizobium leguminosarum bv. trifoliit0symbiont
- Outcome
- phenotypically more similar to each othere4
- Comparator
- isolates of other ETse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rhizobium leguminosarum bv. trifolii”
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e4“BIOLOG substrate utilization patterns showed that isolates within an electrophoretic type (ET) were phenotypically more similar to each other than to isolates of other ETs”
Claim · model-written summary
Group B was identified as the primary creator of the multilocus disequilibrium in the soil population.
- Material
- group Be5
- Interactor
- Rhizobium leguminosarum bv. trifoliiRhizobium leguminosarum bv. trifoliit0symbiont
- Outcome
- primary creator of the disequilibriume5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rhizobium leguminosarum bv. trifolii”
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e5“Evidence for multilocus structure within the population was obtained, and group B was identified as the primary creator of the disequilibrium”
Claim · model-written summary
72 of 75 isolates of the nodule-dominant AS6 complex were found in group B.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e6“Of 75 isolates belonging to the nodule-dominant serotype AS6 complex, 72 were found in group B”
Claim · model-written summary
Subclover isolates WS2-01 and WS2-02 from another Oregon site were also found in group B.
Verbatim
e7“Isolates WS2-01 and WS2-02 representing nodule-dominant serotypes recovered from subclover grown at another Oregon site were also found in group B”
Claim · model-written summary
The most numerous group B electrophoretic types 2 and 3 were suboptimally effective or ineffective at fixing nitrogen on six clover species.
- Material
- ETs 2 and 3e1
- Interactor
- Rhizobium leguminosarum bv. trifoliiRhizobium leguminosarum bv. trifoliit0symbiont
- Outcome
- fixing nitrogene1
- Qualifier
- suboptimally effective or completely ineffectivee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rhizobium leguminosarum bv. trifolii”
e1“Isolates representing the most numerous ETs in group B (ETs 2 and 3) were either suboptimally effective or completely ineffective at fixing nitrogen on six different clover species”
Claim · model-written summary
Four further groups of isolates were identified from 32 strains of diverse origins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e2“Another four groups of isolates (groups A, C, D, and F) were identified when 32 strains of diverse origins were analyzed by MLEE and incorporated into the cluster analysis”
Claim · model-written summary
Group A was the most dissimilar group and contained strain USDA 2124 (T24) with unique symbiotic characteristics.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These isolates represented 13 antigenically distinct serotypes recovered from nodules formed on various clover species”
e2“Group A was most dissimilar in comparisons with other groups and contained strain USDA 2124 (T24), which produces trifolitoxin and has unique symbiotic characteristics”