Abstract
The presence of internal contamination in banana shoot tip cultures has been demonstrated by indexing explants on bacteriological medium. The test involves streaking of the basal part of the shoot tip on to nutrient agar, enriched with yeast extract (5 g 1 -1 ) and glucose (10 g 1 -1 ). 38 bacterial strains were isolated from 851 banana accessions, maintained between 3 and 15 years in the IPGRI/INIBAP in vitro Musa collection. Isolates were identified as Gram-positive endospore forming Bacillus sp. (65 %) and slow growing Mycobacterium sp. (30 %). The selection of an alternative culture medium, Middlebrook 7H10, which meets the nutritional requirements of these mycobacteria made it possible to detect and grow these contaminants more rapidly, Two approaches to decontaminate in vitro cultures have been investigated. The first one involved the use of antibiotics in shoot tip cultures. Ten antibiotics with activity against Gram-positive bacteria were tested against predominant Bacillus sp. and Mycobacterium sp. to determine their minimal bactericidal concentration (MBC). Rifampicin (100 mg 1 -1 ) added in liquid culture was found suitable for controlling Bacillus sp. and Mycobacterium sp. in banana shoot tips, without causing phytotoxic effects. The second approach involved isolation of small explants. Culturing of 1 mm meristems from contaminated in vitro plants or from contaminated plants grown in a greenhouse proved to be effective in eliminating bacteria. Because of the relative simplicity of the technique, meristem culture has been adopted routinely to control bacterial contamination in the banana collection.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Internal bacterial contamination was demonstrated in banana shoot tip cultures.
Verbatim
e0“The presence of internal contamination in banana shoot tip cultures has been demonstrated by indexing explants on bacteriological medium”
Claim · model-written summary
Thirty-eight bacterial strains were isolated from 851 banana accessions.
Verbatim
e1“38 bacterial strains were isolated from 851 banana accessions”
Claim · model-written summary
Bacillus sp. made up 65% of the isolates from banana in vitro cultures.
Verbatim
e0“The presence of internal contamination in banana shoot tip cultures has been demonstrated by indexing explants on bacteriological medium”
e2“Isolates were identified as Gram-positive endospore forming Bacillus sp. (65 %) and slow growing Mycobacterium sp. (30 %)”
Claim · model-written summary
Mycobacterium sp. made up 30% of the isolates from banana in vitro cultures.
- Taxon
- bananae0
- Interactor
- Mycobacterium sp.
Mycobacteriume2other - Outcome
- Isolatese2
- Value
- 30 %e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of internal contamination in banana shoot tip cultures has been demonstrated by indexing explants on bacteriological medium”
e2“Isolates were identified as Gram-positive endospore forming Bacillus sp. (65 %) and slow growing Mycobacterium sp. (30 %)”
Claim · model-written summary
Middlebrook 7H10 medium allowed the mycobacterial contaminants of banana cultures to be detected and grown more rapidly.
- Taxon
- bananae0
- Treatment
- Middlebrook 7H10e3
- Interactor
- mycobacteriae3other
- Outcome
- detect and grow these contaminantse3
- Direction
- more rapidlye3
Schema note from the model: no comparator is named in the abstract for the more rapid detection
Verbatim
e0“The presence of internal contamination in banana shoot tip cultures has been demonstrated by indexing explants on bacteriological medium”
e3“The selection of an alternative culture medium, Middlebrook 7H10, which meets the nutritional requirements of these mycobacteria made it possible to detect and grow these contaminants more rapidly”
Claim · model-written summary
Rifampicin added in liquid culture was suitable for controlling Bacillus sp. and Mycobacterium sp. in banana shoot tips without phytotoxic effects.
- Taxon
- bananae4
- Treatment
- Rifampicin (100 mg 1 -1) added in liquid culturee4
- Interactor
- Bacillus sp.e4other
- Second interactor
- Mycobacterium sp.Mycobacteriume4other
- Outcome
- controlling Bacillus sp. and Mycobacterium sp. in banana shoot tipse4
- Qualifier
- without causing phytotoxic effectse4
- Comparator
- Ten antibioticse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Schema note from the model: comparator is the other antibiotics tested, which the abstract does not name separately
Verbatim
e4“Rifampicin (100 mg 1 -1) added in liquid culture was found suitable for controlling Bacillus sp. and Mycobacterium sp. in banana shoot tips, without causing phytotoxic effects”
e5“Ten antibiotics with activity against Gram-positive bacteria were tested against predominant Bacillus sp. and Mycobacterium sp.”
Claim · model-written summary
Culturing 1 mm meristems from contaminated plants eliminated bacteria from banana cultures.
- Taxon
- bananae7
- Treatment
- 1 mm meristemse6
- Outcome
- eliminating bacteriae6
- Comparator
- contaminated in vitro plantse6
Not reportedDirection
Verbatim
e6“Culturing of 1 mm meristems from contaminated in vitro plants or from contaminated plants grown in a greenhouse proved to be effective in eliminating bacteria”
e7“meristem culture has been adopted routinely to control bacterial contamination in the banana collection”