Abstract
The grape genome is relatively small (approximately 500Mbp) making it an attractive target for genomics. The grape genome is thus similar in size to the rice genome but is composed of a larger number of small chromosomes amenable to physical mapping. Grapes are a woody perennial and thus differ from other species that have been analyzed in detail. We have sequenced cDNA libraries from vegetative tissues and fruits to generate an EST database. Analysis of the first 5000 sequences (2500 from each library) indicated that most ESTs obtained have homology to known genes. The clones had homology to plant genes (68%) and to genes from other organisms (14%). Approximately 18% did not show homology to any sequence of known function in the databases. The two libraries had 275 genes in common. Redundancy was 42% in the leaf library and 39% in the berry library. Functional analysis may identify genes controlling many unique traits in this.
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Claims 7
Claim · model-written summary
About 68% of the grape EST clones had homology to plant genes.
Verbatim
e0“Grapes are a woody perennial”
e1“The clones had homology to plant genes (68%) and to genes from other organisms (14%)”
Claim · model-written summary
About 14% of the grape EST clones had homology to genes from other organisms.
Verbatim
e0“Grapes are a woody perennial”
e1“The clones had homology to plant genes (68%) and to genes from other organisms (14%)”
Claim · model-written summary
About 18% of the grape ESTs did not show homology to any sequence of known function.
Verbatim
e0“Grapes are a woody perennial”
e2“Approximately 18% did not show homology to any sequence of known function in the databases”
Claim · model-written summary
The leaf and berry EST libraries share 275 genes.
Verbatim
e0“Grapes are a woody perennial”
e3“The two libraries had 275 genes in common”
Claim · model-written summary
Redundancy of the grape EST leaf library is 42%.
Verbatim
e0“Grapes are a woody perennial”
e4“Redundancy was 42% in the leaf library and 39% in the berry library”
Claim · model-written summary
Redundancy of the grape EST berry library is 39%.
Verbatim
e0“Grapes are a woody perennial”
e4“Redundancy was 42% in the leaf library and 39% in the berry library”
Claim · model-written summary
Most grape ESTs obtained from the first 5000 sequences have homology to known genes.
Verbatim
e0“Grapes are a woody perennial”
e5“indicated that most ESTs obtained have homology to known genes”