Abstract
In vitro binding assays were used to study the possible role of a cell wall agglutinin in the attachment to plant cell walls of avirulent strains of the wilt pathogen, Pseudomonas solanacearum. In a nitrocellulose filter assay, radioactively labeled lipopolysaccharide (LPS) from the virulent strain, K60, and the avirulent strain, B1, and extracellular polysaccharide (EPS) from K60 were bound quantitatively by the agglutinin extracted from Katahdin potato tubers. The LPS from B1 had significantly greater agglutinin-binding affinity than that from K60 but not after treatment with deoxycholate, which improved solubility. Highly purified chitotetraose did not inhibit binding of K60 LPS to agglutinin, but binding was inhibited by EPS as well as by diverse anionic polymers (DNA, dextran sulfate, xanthan). Binding of agglutinin to EPS and LPS was inhibited at ionic strengths greater than 0.03 and 0.15 M, respectively. It was concluded that electrostatic charge-charge interactions could account for binding of LPS and EPS to potato agglutinin.
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Claims 13
Claim · model-written summary
lipopolysaccharide (LPS) from K60 bound quantitatively to potato tuber agglutinin.
- Taxon
- Potato
Solanum tuberosumt0 - Material
- K60e0
- Organ / stage
- tuberse0
- Gene
- agglutinine0
- Outcome
- lipopolysaccharide (LPS)e0
Not reportedInteractorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“radioactively labeled lipopolysaccharide (LPS) from the virulent strain, K60, and the avirulent strain, B1, and extracellular polysaccharide (EPS) from K60 were bound quantitatively by the agglutinin extracted from Katahdin potato tubers”
Claim · model-written summary
lipopolysaccharide (LPS) from B1 bound quantitatively to potato tuber agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- B1e0
- Organ / stage
- tuberse0
- Gene
- agglutinine0
- Outcome
- lipopolysaccharide (LPS)e0
Not reportedInteractorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“radioactively labeled lipopolysaccharide (LPS) from the virulent strain, K60, and the avirulent strain, B1, and extracellular polysaccharide (EPS) from K60 were bound quantitatively by the agglutinin extracted from Katahdin potato tubers”
Claim · model-written summary
extracellular polysaccharide (EPS) from K60 bound quantitatively to potato tuber agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- K60e0
- Organ / stage
- tuberse0
- Gene
- agglutinine0
- Outcome
- extracellular polysaccharide (EPS)e0
Not reportedInteractorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“radioactively labeled lipopolysaccharide (LPS) from the virulent strain, K60, and the avirulent strain, B1, and extracellular polysaccharide (EPS) from K60 were bound quantitatively by the agglutinin extracted from Katahdin potato tubers”
Claim · model-written summary
B1 LPS had greater agglutinin binding affinity than K60 LPS before deoxycholate treatment.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- B1e0
- Gene
- agglutinine0
- Outcome
- agglutinin-binding affinitye0
- Direction
- greatere0
- Comparator
- K60e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“The LPS from B1 had significantly greater agglutinin-binding affinity than that from K60 but not after treatment with deoxycholate, which improved solubility”
Claim · model-written summary
The B1 versus K60 LPS binding difference disappeared after deoxycholate treatment.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- treatment with deoxycholatee0
- Outcome
- agglutinin-binding affinitye0
- Comparator
- K60e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“The LPS from B1 had significantly greater agglutinin-binding affinity than that from K60 but not after treatment with deoxycholate, which improved solubility”
Claim · model-written summary
chitotetraose did not inhibit K60 LPS binding to potato agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- chitotetraosee0
- Outcome
- binding of K60 LPS to agglutinine0
- Direction
- did not inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Highly purified chitotetraose did not inhibit binding of K60 LPS to agglutinin, but binding was inhibited by EPS as well as by diverse anionic polymers (DNA, dextran sulfate, xanthan)”
Claim · model-written summary
EPS inhibited K60 LPS binding to potato agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- EPSe0
- Outcome
- binding of K60 LPS to agglutinine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Highly purified chitotetraose did not inhibit binding of K60 LPS to agglutinin, but binding was inhibited by EPS as well as by diverse anionic polymers (DNA, dextran sulfate, xanthan)”
Claim · model-written summary
DNA inhibited K60 LPS binding to potato agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- DNAe0
- Outcome
- binding of K60 LPS to agglutinine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Highly purified chitotetraose did not inhibit binding of K60 LPS to agglutinin, but binding was inhibited by EPS as well as by diverse anionic polymers (DNA, dextran sulfate, xanthan)”
Claim · model-written summary
dextran sulfate inhibited K60 LPS binding to potato agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- dextran sulfatee0
- Outcome
- binding of K60 LPS to agglutinine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Highly purified chitotetraose did not inhibit binding of K60 LPS to agglutinin, but binding was inhibited by EPS as well as by diverse anionic polymers (DNA, dextran sulfate, xanthan)”
Claim · model-written summary
xanthan inhibited K60 LPS binding to potato agglutinin.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- xanthane0
- Outcome
- binding of K60 LPS to agglutinine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Highly purified chitotetraose did not inhibit binding of K60 LPS to agglutinin, but binding was inhibited by EPS as well as by diverse anionic polymers (DNA, dextran sulfate, xanthan)”
Claim · model-written summary
Agglutinin binding to EPS was inhibited at ionic strength greater than 0.03.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- ionic strengthse0
- Outcome
- agglutinin to EPS and LPSe0
- Direction
- inhibitede0
- Value
- 0.03e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Binding of agglutinin to EPS and LPS was inhibited at ionic strengths greater than 0.03 and 0.15 M, respectively”
Claim · model-written summary
Agglutinin binding to LPS was inhibited at ionic strength greater than 0.15 M.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- ionic strengthse0
- Outcome
- agglutinin to EPS and LPSe0
- Direction
- inhibitede0
- Value
- 0.15 Me0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Binding of agglutinin to EPS and LPS was inhibited at ionic strengths greater than 0.03 and 0.15 M, respectively”
Claim · model-written summary
The authors proposed electrostatic charge interactions as an explanation for LPS and EPS binding to potato agglutinin.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- binding of LPS and EPS to potato agglutinine0
- Qualifier
- electrostatic charge-charge interactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“electrostatic charge-charge interactions could account for binding of LPS and EPS to potato agglutinin”
The same questions in other papers
polysaccharide · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.