Abstract
A new isolate of grapevine leafroll-associated virus 2 (GLRaV-2-H4) was recovered by mechanically inoculating herbaceous hosts with concentrated tissue extracts from a North American accession of Vitis rupestris. Contrary to the Semillon isolate of GLRaV-2, isolate H4 elicited necrotic local lesions in Nicotiana clevelandii and infected systemically N. occidentalis inducing very severe symptoms. The migration rate of dissociated capsid protein of GLRaV-2-H4 in SDS-PAGE differed slightly from that of GLRaV-2-Sem. The coat protein sequence of GLRaV-2-H4 differed by about 12 % at the nucleotide level from capsid proteins of the other two GLRaV-2 isolates that have been sequenced to date. No serological differences could be detected. Isolate H4 is a biological variant of GLRaV-2, which can be distinguished from other mechanically transmitted isolates of the same virus because of differences in type and reactions of the herbaceous host range and in molecular traits of the coat protein cistron.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
H4 produced necrotic local lesions in Nicotiana clevelandii unlike the Semillon isolate.
- Taxon
- Nicotiana clevelandii
Nicotiana clevelandiie0 - Interactor
- GLRaV-2e0pathogen
- Outcome
- necrotic local lesionse0
- Comparator
- Semillon isolatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Contrary to the Semillon isolate of GLRaV-2, isolate H4 elicited necrotic local lesions in Nicotiana clevelandii”
Claim · model-written summary
H4 systemically infected N. occidentalis with very severe symptoms.
- Taxon
- N. occidentalis
Nicotiana occidentalise0 - Interactor
- isolate H4e0pathogen
- Outcome
- infected systemically N. occidentalis inducing very severe symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolate H4 elicited necrotic local lesions in Nicotiana clevelandii and infected systemically N. occidentalis inducing very severe symptoms.”
Claim · model-written summary
H4 capsid protein migrated slightly differently from GLRaV-2-Sem protein.
- Interactor
- GLRaV-2-H4e0pathogen
- Gene
- capsid proteine0
- Outcome
- migration rate of dissociated capsid proteine0
- Direction
- differed slightlye0
- Comparator
- GLRaV-2-Seme0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The migration rate of dissociated capsid protein of GLRaV-2-H4 in SDS-PAGE differed slightly from that of GLRaV-2-Sem.”
t0“grapevine”
Claim · model-written summary
H4 coat protein sequence differed by about 12 % from two sequenced isolates.
- Interactor
- GLRaV-2-H4e0pathogen
- Gene
- coat proteine0
- Outcome
- coat protein sequencee0
- Direction
- differede0
- Value
- about 12 %e0
- Comparator
- the other two GLRaV-2 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The coat protein sequence of GLRaV-2-H4 differed by about 12 % at the nucleotide level from capsid proteins of the other two GLRaV-2 isolates that have been sequenced to date.”
t0“grapevine”
Claim · model-written summary
No serological differences were detected.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No serological differences could be detected.”
t0“grapevine”
Claim · model-written summary
H4 was classified as a biological variant distinguishable through host reactions and coat protein traits.
- Interactor
- GLRaV-2e0pathogen
- Outcome
- biological variant of GLRaV-2e0
- Qualifier
- differences in type and reactions of the herbaceous host range and in molecular traits of the coat protein cistrone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolate H4 is a biological variant of GLRaV-2, which can be distinguished from other mechanically transmitted isolates of the same virus because of differences in type and reactions of the herbaceous host range and in molecular traits of the coat protein cistron.”
t0“grapevine”