Abstract
The virus isolate Okra06 was obtained from the diseased okra plants exhibiting yellow vein and leaf curl symptoms in Guangzhou,Guangdong Province.The results of PCR detection indicated that the virus belonged to the genus Begomovirus of the family Geminiviridae.The genome of the virus was cloned and sequenced.The results showed that it had only A component(DNA-A).The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides,encoding six potential ORFs.The comparison showed that Okra06 DNA-A had the highest nucleotide sequence identity(99.7%) with CLCuMV-.Six ORFs shared 100%,100%,99.6%,99.8%,100% and 99.7% nucleotide sequence identities with CLCuMV – ,respectively.The Okra06 was also associated with satellite DNA β molecular.The full-length sequence of DNA β was determined to be 1 346 nucleotides,encoding one potential ORF(C1).The comparison further showed that Okra06 DNA β had the highest nucleotide sequence identity(99.5%)with G6 DNA β.Phylogenetic analysis revealed that DNA β of isolate Okra06,G6 and Gx08 were more closely related than the other begomoviruses associated with DNA β,and were clustered in a separate branch and further clustered with other isolates of CLCuMV DNA β or CLCuV DNA β.Those results revealed that the virus isolate Okra06 infecting okra plants in Guangdong belonged to the species CLCuMV,and it should be the Hibiscus strain.
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Claims 9
Claim · model-written summary
The virus from symptomatic okra belonged to Begomovirus in Geminiviridae.
- Interactor
- Begomovirus
Begomoviruse0pathogen - Outcome
- belonged to the genus Begomoviruse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus belonged to the genus Begomovirus of the family Geminiviridae”
Claim · model-written summary
Okra06 had only a DNA-A component of 2 737 nucleotides encoding six potential ORFs.
- Gene
- DNA-Ae0
- Outcome
- complete nucleotide sequencee0
- Value
- 2 737 nucleotidese0
- Qualifier
- six potential ORFse0
Not reportedTaxon
Verbatim
e0“only A component(DNA-A).The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides,encoding six potential ORFs”
Claim · model-written summary
Okra06 DNA-A had 99.7% nucleotide sequence identity to CLCuMV.
Not reportedTaxon
Verbatim
e0“Okra06 DNA-A had the highest nucleotide sequence identity(99.7%) with CLCuMV-”
Claim · model-written summary
Six Okra06 ORFs had reported nucleotide sequence identities to CLCuMV.
Not reportedTaxon
Verbatim
e0“Six ORFs shared 100%,100%,99.6%,99.8%,100% and 99.7% nucleotide sequence identities with CLCuMV”
Claim · model-written summary
Okra06 was associated with satellite DNA β.
Not reportedTaxon
Verbatim
e0“Okra06 was also associated with satellite DNA β molecular”
Claim · model-written summary
Okra06 DNA β had 1 346 nucleotides and one potential ORF.
Not reportedTaxon
Verbatim
e0“full-length sequence of DNA β was determined to be 1 346 nucleotides,encoding one potential ORF(C1)”
Claim · model-written summary
Okra06 DNA β had 99.5% sequence identity to G6 DNA β.
Not reportedTaxon
Verbatim
e0“Okra06 DNA β had the highest nucleotide sequence identity(99.5%)with G6 DNA β”
Claim · model-written summary
Phylogenetic analysis placed Okra06, G6 and Gx08 DNA β close together in a separate branch.
Not reportedTaxon
Verbatim
e0“DNA β of isolate Okra06,G6 and Gx08 were more closely related than the other begomoviruses associated with DNA β,and were clustered in a separate branch”
Claim · model-written summary
The okra-infecting Okra06 isolate was identified as CLCuMV Hibiscus strain.
- Taxon
- okra
.jpg?width=60)
Abelmoschus esculentuse0 - Interactor
- CLCuMVe0pathogen
- Outcome
- Hibiscus straine0
- Setting
- Guangdonge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“virus isolate Okra06 infecting okra plants in Guangdong belonged to the species CLCuMV,and it should be the Hibiscus strain”