Abstract
In all Romanian national fruit collections, all accessions holding a phenotypic, biochemical and genetic evaluation, but less a molecular level assessment of their….. For introduction of this material (accessions) into the breeding programs is necessary and important to know the level of characterization. Considering this fact, in the present study was optimization of the PCR protocol, specially annealing temperature of fifteen SSR primer pairs to characterize and study the genetic similarity and to determine the polymorphism between 36 sweet cherry accessions from I.C.D.P. Iasi, Romania. In the optimization process of annealing temperature for primers, was started with 5oC less them the temperature recommendated by the manufacturer, gradually ascending with 1-2oC/PCR cycle, up to 10-11oC, above the recommended value. For every primers pairs it was 6-7 steps for optimization of annealing temperature, obtaining satisfactory results to the following primers pairs: UCDCH17, UDP96-001 and PceGA25 at 65oC, UCDCH21: 64oC, UCDCH31: 66oC,:UPD97-402: 60oC, PMS3: 68oC. The primer pairs UDP96-008 obtained at 65oC, monomorphic bands, so it was excluded from the analysis. The other reagents used in PCR and temperature regimes (denaturation and extension temperature) were kept constant. From these results we conclude the transferability and applicability of SSR markers for genotyping and phylogenetic studies in the genus Prunus.
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Claims 7
Claim · model-written summary
SSR primers UCDCH17, UDP96-001 and PceGA25 gave satisfactory amplification at 65oC in sweet cherry.
- Taxon
- Sweet Cherry
Prunus aviumt0 - Outcome
- satisfactory resultse1
- Value
- 65oCe1
- Qualifier
- UCDCH17, UDP96-001 and PceGA25e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Cherry Cultivars”
e1“obtaining satisfactory results to the following primers pairs: UCDCH17, UDP96-001 and PceGA25 at 65oC, UCDCH21: 64oC, UCDCH31: 66oC,:UPD97-402: 60oC, PMS3: 68oC”
Claim · model-written summary
SSR primer UDP96-008 gave monomorphic bands at 65oC in sweet cherry, so it was excluded from the analysis.
- Taxon
- Sweet CherryPrunus aviumt0
- Outcome
- monomorphic bandse2
- Value
- 65oCe2
- Qualifier
- excluded from the analysise2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Cherry Cultivars”
e2“The primer pairs UDP96-008 obtained at 65oC, monomorphic bands, so it was excluded from the analysis”
Claim · model-written summary
SSR primer UCDCH21 gave satisfactory amplification at 64oC in sweet cherry.
- Taxon
- Sweet CherryPrunus aviumt0
- Outcome
- satisfactory resultse1
- Value
- 64oCe1
- Qualifier
- UCDCH21e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Cherry Cultivars”
e1“obtaining satisfactory results to the following primers pairs: UCDCH17, UDP96-001 and PceGA25 at 65oC, UCDCH21: 64oC, UCDCH31: 66oC,:UPD97-402: 60oC, PMS3: 68oC”
Claim · model-written summary
SSR primer UCDCH31 gave satisfactory amplification at 66oC in sweet cherry.
- Taxon
- Sweet CherryPrunus aviumt0
- Outcome
- satisfactory resultse1
- Value
- 66oCe1
- Qualifier
- UCDCH31e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Cherry Cultivars”
e1“obtaining satisfactory results to the following primers pairs: UCDCH17, UDP96-001 and PceGA25 at 65oC, UCDCH21: 64oC, UCDCH31: 66oC,:UPD97-402: 60oC, PMS3: 68oC”
Claim · model-written summary
SSR primer UPD97-402 gave satisfactory amplification at 60oC in sweet cherry.
- Taxon
- Sweet CherryPrunus aviumt0
- Outcome
- satisfactory resultse1
- Value
- 60oCe1
- Qualifier
- UPD97-402e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Cherry Cultivars”
e1“obtaining satisfactory results to the following primers pairs: UCDCH17, UDP96-001 and PceGA25 at 65oC, UCDCH21: 64oC, UCDCH31: 66oC,:UPD97-402: 60oC, PMS3: 68oC”
Claim · model-written summary
SSR primer PMS3 gave satisfactory amplification at 68oC in sweet cherry.
- Taxon
- Sweet CherryPrunus aviumt0
- Outcome
- satisfactory resultse1
- Value
- 68oCe1
- Qualifier
- PMS3e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Cherry Cultivars”
e1“obtaining satisfactory results to the following primers pairs: UCDCH17, UDP96-001 and PceGA25 at 65oC, UCDCH21: 64oC, UCDCH31: 66oC,:UPD97-402: 60oC, PMS3: 68oC”
Claim · model-written summary
SSR markers are transferable and applicable for genotyping and phylogenetic studies in Prunus.
- Outcome
- transferability and applicability of SSR markerse7
PrunusNot reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“transferability and applicability of SSR markers for genotyping and phylogenetic studies in the genus Prunus”