Abstract
The control of flowering time requires activation of a cascade of successive genes which are affected by several internal and external factors. Some of these genes, such as Curly Leaf (CLF), have inhibitory effect on flowering. Thus, by silencing of these genes, initiation of flowering can be accelerated. In this research, the RNA Silencing technology is used to investigate the possibility of production of early-flowering plants via CLF silencing. First, in order to produce hairpin structure, a fragment of CLF gene of Arabidopsis thaliana was amplified by PCR and then cloned in two sense and antisense directions, into pFGC5941 vector. The resulted recombinant vector was then transferred into Arabidopsis plants via Agrobacterium method. As expected, initiation of flowering was accelerated in transgenic plants in comparison with the wild-type plants. In agreement to the obtained results, RT-qPCR analysis showed that CLF expression in transgenic plants was decreased in comparison with the wild-type plants. In contrast, FT (a gene which induces flowering) expression was increased in transgenic plants. These results show that RNA silencing as a novel and time-saving biotechnological method could be applied to generate early-flowering plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Flowering was accelerated in CLF-silenced transgenic Arabidopsis plants compared with wild-type plants.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- transgenic plantse2
- Treatment
- CLF silencinge1
- Gene
- CLFe1
- Outcome
- initiation of floweringe2
- Direction
- acceleratede2
- Comparator
- wild-type plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“As expected, initiation of flowering was accelerated in transgenic plants in comparison with the wild-type plants”
e1“In this research, the RNA Silencing technology is used to investigate the possibility of production of early-flowering plants via CLF silencing”
t0“Generation of Arabidopsis early flowering plants by destruction of CLF transcripts via RNA silencing”
Claim · model-written summary
CLF expression was decreased in transgenic Arabidopsis plants compared with wild-type plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse3
- Treatment
- RNA Silencing technologye1
- Gene
- CLFe3
- Outcome
- CLF expressione3
- Direction
- decreasede3
- Comparator
- wild-type plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In agreement to the obtained results, RT-qPCR analysis showed that CLF expression in transgenic plants was decreased in comparison with the wild-type plants”
e1“In this research, the RNA Silencing technology is used to investigate the possibility of production of early-flowering plants via CLF silencing”
t0“Generation of Arabidopsis early flowering plants by destruction of CLF transcripts via RNA silencing”
Claim · model-written summary
FT expression was increased in transgenic Arabidopsis plants compared with wild-type plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse4
- Treatment
- RNA Silencing technologye1
- Gene
- FTe4
- Outcome
- FT (a gene which induces flowering) expressione4
- Direction
- increasede4
- Comparator
- wild-type plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In contrast, FT (a gene which induces flowering) expression was increased in transgenic plants”
e3“In agreement to the obtained results, RT-qPCR analysis showed that CLF expression in transgenic plants was decreased in comparison with the wild-type plants”
e1“In this research, the RNA Silencing technology is used to investigate the possibility of production of early-flowering plants via CLF silencing”
t0“Generation of Arabidopsis early flowering plants by destruction of CLF transcripts via RNA silencing”
Claim · model-written summary
RNA silencing could be applied to generate early-flowering plants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- RNA silencinge5
- Outcome
- generate early-flowering plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“These results show that RNA silencing as a novel and time-saving biotechnological method could be applied to generate early-flowering plants”
t0“Generation of Arabidopsis early flowering plants by destruction of CLF transcripts via RNA silencing”