Abstract
Avocado (Persea americana) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at pH 7.4 and under conditions mimicking reverse-phase capture at pH 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low-abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein, a glucanase, and an isoflavone reductase like protein.
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Claims 7
Claim · model-written summary
The avocado pulp proteome comprises 1012 unique gene products.
- Taxon
- avocado
Persea americanat0 - Organ / stage
- pulpt0
- Outcome
- unique gene products identifiede0
- Value
- 1012 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e0“The total number of unique gene products identified amounts to 1012 proteins”
Claim · model-written summary
Combinatorial peptide ligand library capture detected 648 proteins not seen in the control sample.
- Taxon
- avocadoPersea americanat0
- Outcome
- new species detected via combinatorial peptide ligand librariese1
- Value
- 648e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e1“648 represent the new species detected via combinatorial peptide ligand libraries”
Claim · model-written summary
174 proteins are in common between the treated and control avocado pulp samples.
- Taxon
- avocadoPersea americanat0
- Outcome
- in common with the control, untreated samplee2
- Value
- 174e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e2“174 are in common with the control, untreated sample”
Claim · model-written summary
190 proteins are present only in the control avocado pulp sample.
- Taxon
- avocadoPersea americanat0
- Outcome
- present only in the controle3
- Value
- 190e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e3“190 are present only in the control”
Claim · model-written summary
The known avocado allergen Pers a 1 was identified in the pulp proteome.
- Taxon
- avocadoPersea americanat0
- Gene
- Pers a 1e4
- Outcome
- identify the already known avocado allergene4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e4“it was possible to identify the already known avocado allergen Pers a 1”
Claim · model-written summary
Profilin, polygalacturonase and thaumatin-like protein were identified as proteins susceptible to be allergens.
- Taxon
- avocadoPersea americanat0
- Gene
- a profilin, a polygalacturonase, a thaumatin-like proteine5
- Outcome
- susceptible to be allergense5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e5“different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein”
Claim · model-written summary
Glucanase and isoflavone reductase like protein were identified as proteins susceptible to be allergens.
- Taxon
- avocadoPersea americanat0
- Gene
- a glucanase, and an isoflavone reductase like proteine6
- Outcome
- susceptible to be allergense5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of avocado (Persea americana) pulp proteins”
e5“different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein”
e6“a glucanase, and an isoflavone reductase like protein”