Abstract
A simple method to measure the degree of methyl esterification in small samples of pectins or isolated cell walls will be described. The method involves selective reduction of methyl esterified galacturonic acid to galactose with sodium borohydride or sodium borodeuteride in the presence of strong buffer at 4 C. Quantitative reduction of samples can be accomplished in 1 h using 20 mg borohydride per mg sample. The degree of pectin methyl esterification can then be determined by measuring an increase of galactose using gas chromatography (borohydride reduced samples) or gas chromatography/mass spectroscopy (borodeuteride reduced samples), or by measuring the decrease in galacturonic acid using conventional colorimetric methods. Pectin samples as small as 50 {mu}g have been analyzed using the reduction method with good results.
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Claims 5
Claim · model-written summary
Quantitative reduction of samples can be accomplished in 1 h with 20 mg borohydride per mg sample.
Not reportedTaxon
Verbatim
e2“Quantitative reduction of samples can be accomplished in 1 h using 20 mg borohydride per mg sample”
Claim · model-written summary
Pectin samples as small as 50 {mu}g were analysed with the reduction method with good results.
Not reportedTaxon
Verbatim
e5“Pectin samples as small as 50 {mu}g have been analyzed using the reduction method with good results”
Claim · model-written summary
Degree of pectin methyl esterification can be determined by gas chromatography, measuring an increase of galactose (borohydride reduced samples).
- Outcome
- increase of galactosee4
- Direction
- increasee4
- Qualifier
- gas chromatography (borohydride reduced samples)e4
- Comparator
- conventional colorimetric methodse4
Not reportedTaxon
Verbatim
e4“The degree of pectin methyl esterification can then be determined by measuring an increase of galactose using gas chromatography (borohydride reduced samples) or gas chromatography/mass spectroscopy (borodeuteride reduced samples), or by measuring the decrease in galacturonic acid using conventional colorimetric methods”
Claim · model-written summary
Degree of pectin methyl esterification can be determined by measuring a decrease in galacturonic acid with conventional colorimetric methods.
- Outcome
- decrease in galacturonic acide4
- Direction
- decreasee4
- Qualifier
- conventional colorimetric methodse4
- Comparator
- gas chromatographye4
Not reportedTaxon
Verbatim
e4“The degree of pectin methyl esterification can then be determined by measuring an increase of galactose using gas chromatography (borohydride reduced samples) or gas chromatography/mass spectroscopy (borodeuteride reduced samples), or by measuring the decrease in galacturonic acid using conventional colorimetric methods”
Claim · model-written summary
Selective reduction of methyl esterified galacturonic acid to galactose is used with sodium borohydride or sodium borodeuteride in strong buffer at 4 C.
- Treatment
- sodium borohydridee1
- Outcome
- selective reduction of methyl esterified galacturonic acid to galactosee1
- Qualifier
- strong buffer at 4 Ce1
- Comparator
- sodium borodeuteridee1
Not reportedTaxon
Verbatim
e1“The method involves selective reduction of methyl esterified galacturonic acid to galactose with sodium borohydride or sodium borodeuteride in the presence of strong buffer at 4 C”