Abstract
Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/ of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/ of NAA and 1 mg/ of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/ of 2,4-D, and 3 mg/ of 2,4-D and 3 mg/ of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/ of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly.
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Claims 9
Claim · model-written summary
Percentage of callus induction in oat was significantly affected by combinations of plant growth regulators.
- Taxon
- oat
Avena sativae0 - Treatment
- combinations of plant growth regulatorse1
- Outcome
- Percentage of callus inductione1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e1“Percentage of callus induction showed significant among the combinations of plant growth regulators”
Claim · model-written summary
Callus induction in oat showed high efficiency in medium containing 3 mg/ of 2,4-D.
- Taxon
- oatAvena sativae0
- Treatment
- 3 mg/ of 2,4-De2
- Outcome
- Callus inductione2
- Direction
- highe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e2“Callus induction showed high efficiency in medium containing 3 mg/ of 2,4-D”
Claim · model-written summary
Genotype Gwiri37 had a high frequency of callus induction in oat.
- Taxon
- oatAvena sativae0
- Material
- Gwiri37e3
- Outcome
- frequency of callus inductione3
- Direction
- highe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e3“The high frequency of callus induction was obtained in Gwiri37”
Claim · model-written summary
Medium with 0.2 mg/ of NAA and 1 mg/ of BA gave high plant regeneration percentage in oat.
- Taxon
- oatAvena sativae0
- Treatment
- 0.2 mg/ of NAA and 1 mg/ of BAe4
- Outcome
- Percentage of plant regeneratione4
- Direction
- highe4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e4“Percentage of plant regeneration showed high in MS medium containing 0.2 mg/ of NAA and 1 mg/ of BA”
Claim · model-written summary
Callus induction media with 3 mg/ of 2,4-D and 3 mg/ of kinetin gave high frequency of plant regeneration in oat.
- Taxon
- oatAvena sativae0
- Treatment
- 3 mg/ of 2,4-D and 3 mg/ of kinetine5
- Outcome
- frequency for plant regeneratione5
- Direction
- highe5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e5“Treatment with 3 mg/ of 2,4-D, and 3 mg/ of 2,4-D and 3 mg/ of kinetin in callus induction media showed high frequency for plant regeneration”
Claim · model-written summary
Plant regeneration frequency in oat was significantly affected by genotype.
- Taxon
- oatAvena sativae0
- Material
- genotypese6
- Outcome
- Plant regeneration frequencye6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e6“Plant regeneration frequency among the genotypes showed significant”
Claim · model-written summary
Gwiri37 showed high plant regeneration frequency in oat.
- Taxon
- oatAvena sativae0
- Material
- Gwiri37e7
- Outcome
- regeneration frequencye7
- Direction
- highe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e7“Especially, Gwiri37 showed high regeneration frequency”
Claim · model-written summary
IBA treatment of oat shoots induced root formation rapidly.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e2“Treatment of shoots with IBA induced root formation rapidly”
Claim · model-written summary
The callus initiation medium affected subsequent plant regeneration in oat.
- Taxon
- oatAvena sativae0
- Treatment
- callus initiation mediume3
- Outcome
- subsequent plant regeneratione3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration”
e3“The callus initiation medium affected the subsequent plant regeneration”
The same questions in other papers
2,4-D · growth · Avena sativa (oats)Positive and negative results
1 in the opposite direction
- negative
N-benzyladenine · growth · Avena sativa (oats)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
kinetin · growth · Avena sativa (oats)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
1-naphthaleneacetic acid · growth · Avena sativa (oats)Results agree
1 paper in the same direction as this paper
- positive
indole-3-butyric acid · growth · Avena sativa (oats)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.