Paper · 1988 · Plant and Cell Physiology

The Influence of Different Cytokinins and Auxins on Flower Neoformation in Thin Cell Layers of Nicotiana tabacum L.

C. Heylen; J.C. Vendrig

7
claims
15
verbatim quotes
31
citations
2
research areas
—
Not open access

Abstract

Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L. Organogenesis was found to be expressed in different ways according to the kind of auxin and cytokinin present in the culture medium. In the presence of synthetic auxins (NAA, CTA), more flower buds were generated than with the natural auxins (IAA, IBA), if the culture medium was also supplemented with a cytokinin. These conditions also stimulated the development of the buds to flowers or inflorescences. The most active cytokinin was BA. Natural cytokinins (zeatin, IPA) appeared much less active but led to remarkable development of roots together with flower buds. An organogenetic effect of 2,4-D was observed in this experimental system, leading to the conclusion that organogenetic activity of cytokinins and auxins in thin cell layer cultures strongly depends on the molecular structure of the growth regulators.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

More flower buds were generated with synthetic auxins (NAA, CTA) than with natural auxins (IAA, IBA) when the medium also contained a cytokinin.

Taxon
Nicotiana tabacumNicotiana tabacume0
Organ / stage
thin cell layerse0
Treatment
synthetic auxins (NAA, CTA)e1
Outcome
flower budse1
Direction
moree1
Qualifier
if the culture medium was also supplemented with a cytokinine1
Comparator
natural auxins (IAA, IBA)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e1

“In the presence of synthetic auxins (NAA, CTA), more flower buds were generated than with the natural auxins (IAA, IBA), if the culture medium was also supplemented with a cytokinin”

✓ located verbatim in the source · abstract, characters 247–428 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Synthetic auxins with a cytokinin stimulated the development of buds into flowers or inflorescences.

Taxon
Nicotiana tabacumNicotiana tabacume0
Treatment
synthetic auxins (NAA, CTA)e1
Outcome
development of the buds to flowers or inflorescencese2
Direction
stimulatede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e1

“In the presence of synthetic auxins (NAA, CTA), more flower buds were generated than with the natural auxins (IAA, IBA), if the culture medium was also supplemented with a cytokinin”

✓ located verbatim in the source · abstract, characters 247–428 · show in text ↑
e2

“These conditions also stimulated the development of the buds to flowers or inflorescences”

✓ located verbatim in the source · abstract, characters 430–519 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

BA was the most active cytokinin.

Taxon
Nicotiana tabacumNicotiana tabacume0
Treatment
BAe3
Outcome
most active cytokinine3
Direction
most activee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e3

“The most active cytokinin was BA”

✓ located verbatim in the source · abstract, characters 521–553 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Natural cytokinins (zeatin, IPA) were much less active.

Taxon
Nicotiana tabacumNicotiana tabacume0
Treatment
Natural cytokinins (zeatin, IPA)e4
Outcome
activee4
Direction
much lesse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e4

“Natural cytokinins (zeatin, IPA) appeared much less active but led to remarkable development of roots together with flower buds”

✓ located verbatim in the source · abstract, characters 555–682 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Natural cytokinins (zeatin, IPA) led to remarkable development of roots.

Taxon
Nicotiana tabacumNicotiana tabacume0
Treatment
Natural cytokinins (zeatin, IPA)e4
Outcome
development of rootse4
Direction
remarkable developmente4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e4

“Natural cytokinins (zeatin, IPA) appeared much less active but led to remarkable development of roots together with flower buds”

✓ located verbatim in the source · abstract, characters 555–682 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

An organogenetic effect of 2,4-D was observed in the tobacco thin cell layer system.

Taxon
Nicotiana tabacumNicotiana tabacume0
Treatment
2,4-De5
Outcome
organogenetic effecte5

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e5

“An organogenetic effect of 2,4-D was observed in this experimental system”

✓ located verbatim in the source · abstract, characters 684–757 · show in text ↑
Associationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Organogenetic activity of cytokinins and auxins in thin cell layer cultures depends strongly on the molecular structure of the growth regulators, as the authors conclude.

Taxon
Nicotiana tabacumNicotiana tabacume0
Treatment
cytokinins and auxinse6
Outcome
organogenetic activitye6
Qualifier
molecular structure of the growth regulatorse6

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Neoformation of flower buds was studied in thin cell layers derived from floral branches of Nicotiana tabacum L”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e6

“organogenetic activity of cytokinins and auxins in thin cell layer cultures strongly depends on the molecular structure of the growth regulators”

✓ located verbatim in the source · abstract, characters 790–934 · show in text ↑

synthetic auxin · growth · Nicotiana tabacum (tobacco)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

1-naphthaleneacetic acid · growth · Nicotiana tabacum (tobacco)Results agree

10 papers: 10 positive, 0 negative or no effect

9 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.