Abstract
ABSTRACT The self-incompatibility (S) genotypes of a collection of 65 Belgian accessions of wild cherry, selected within two populations and planted in a seed orchard, were determined using polymerase chain reaction (PCR) methods. Initially, DNA extracts were amplified with consensus primers that amplify across the second intron of the S-ribonuclease gene which shows considerable length polymorphism. The provisional genotypes deduced were checked with the appropriate allele-specific primers for the known alleles S(1) to S(16). Putative new alleles were subjected to PCR with consensus primers amplifying across the first intron. Six new alleles, S(17) to S(22), were thus indicated on the basis of the estimated lengths of the first and second intron PCR products. Examples of these alleles were partially sequenced and were indeed mutually distinct and different from the known alleles. The incompatibility genotypes of all 65 accessions were determined and one triploid individual was found. Seventeen alleles were detected in all. Allele frequencies differed between samples and the expected total number of alleles in the underlying populations was estimated. The wild cherry populations differed significantly with respect to allelic frequencies from sweet cherry cultivars; alleles S(4) and S(5), which are moderately frequent in sweet cherry, were absent from the wild cherry accessions. The knowledge of the S genotypes will be useful for studying the gene flow within the seed orchard and these approaches should also be informative in wild populations.
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Claims 8
Claim · model-written summary
Six previously unreported S alleles were indicated.
Verbatim
e0“Six new alleles, S(17) to S(22), were thus indicated on the basis of the estimated lengths of the first and second intron PCR products.”
t0“wild cherries”
Claim · model-written summary
The putative new alleles were mutually distinct and different from known alleles.
Verbatim
e0“Examples of these alleles were partially sequenced and were indeed mutually distinct and different from the known alleles.”
t0“wild cherries”
Claim · model-written summary
Incompatibility genotypes were determined for all 65 accessions.
Verbatim
e0“The incompatibility genotypes of all 65 accessions were determined and one triploid individual was found.”
t0“wild cherries”
Claim · model-written summary
One triploid wild cherry individual was found.
Verbatim
e0“The incompatibility genotypes of all 65 accessions were determined and one triploid individual was found.”
t0“wild cherries”
Claim · model-written summary
Seventeen alleles were detected.
Verbatim
e0“Seventeen alleles were detected in all.”
t0“wild cherries”
Claim · model-written summary
Allele frequencies differed between samples.
Verbatim
e0“Allele frequencies differed between samples”
t0“wild cherries”
Claim · model-written summary
Wild cherry populations differed significantly in allelic frequencies from sweet cherry cultivars.
Verbatim
e0“The wild cherry populations differed significantly with respect to allelic frequencies from sweet cherry cultivars”
t0“wild cherries”
Claim · model-written summary
S(4) and S(5) were absent from wild cherry accessions.
Verbatim
e0“alleles S(4) and S(5), which are moderately frequent in sweet cherry, were absent from the wild cherry accessions.”