Abstract
Twenty-two new grape microsatellite markers were developed from CT-repeat regions cloned from Vitis vinifera Pinot noir and Cabernet Sauvignon. These loci were amplified in from 51 to 347 cultivars of V. vinifera. Twelve of the markers are polymorphic and informative in V. vinifera. Five are monomorphic in V. vinifera and the remaining five are polymorphic but less useful because they have only two alleles or more than two bands are amplified per individual. The number of alleles detected in the twelve informative markers ranged from 4 to 19, with heterozygosity values ranging from 0.42 to 0.90. Primer sequences and allele frequencies in the cultivars tested are reported for these markers. Revised allele frequencies are also presented for three previously reported microsatellite markers. Genotypes for the 12 informative new markers are presented for seven suggested reference cultivars.
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Claims 9
Claim · model-written summary
Twenty-two new grape microsatellite markers were developed.
- Taxon
- Vitis vinifera
Vitis viniferae0 - Material
- Pinot noire0
- Outcome
- grape microsatellite markerse0
- Value
- Twenty-twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty-two new grape microsatellite markers were developed from CT-repeat regions cloned from Vitis vinifera Pinot noir and Cabernet Sauvignon”
Claim · model-written summary
Twelve markers were polymorphic and informative in V. vinifera.
- Taxon
- V. viniferaVitis viniferae0
- Outcome
- polymorphic and informativee0
- Value
- Twelvee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve of the markers are polymorphic and informative in V. vinifera”
Claim · model-written summary
Five markers were monomorphic in V. vinifera.
- Taxon
- V. viniferaVitis viniferae0
- Outcome
- monomorphice0
- Value
- Fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five are monomorphic in V. vinifera”
Claim · model-written summary
Five other polymorphic markers had limited usefulness because they had two alleles or amplified more than two bands.
Verbatim
e0“the remaining five are polymorphic but less useful because they have only two alleles or more than two bands are amplified per individual”
t0“Grape”
Claim · model-written summary
The twelve informative markers detected 4 to 19 alleles.
Verbatim
e0“The number of alleles detected in the twelve informative markers ranged from 4 to 19, with heterozygosity values ranging from 0.42 to 0.90”
t0“Grape”
Claim · model-written summary
Heterozygosity of the informative markers ranged from 0.42 to 0.90.
Verbatim
e0“heterozygosity values ranging from 0.42 to 0.90”
t0“Grape”
Claim · model-written summary
Primer sequences and allele frequencies were reported for the new markers.
Not reportedComparator
Verbatim
e0“Primer sequences and allele frequencies in the cultivars tested are reported for these markers”
t0“Grape”
Claim · model-written summary
Revised allele frequencies were presented for three earlier microsatellite markers.
Verbatim
e0“Revised allele frequencies are also presented for three previously reported microsatellite markers”
t0“Grape”
Claim · model-written summary
Genotypes at the twelve informative markers were provided for seven suggested reference cultivars.
Verbatim
e0“Genotypes for the 12 informative new markers are presented for seven suggested reference cultivars”
t0“Grape”