Abstract
The Pseudomonas syringae pathovars are composed of host-specific plant pathogens that characteristically elicit the defense-associated hypersensitive response (HR) in nonhost plants. P. s. pv. syringae 61 secretes an HR elicitor, harpinPss (HrpZPss), in a hrp-dependent manner. An internal fragment of the P. s. pv. syringae 61 hrpZ gene was used to clone the hrpZ locus from P. s. pv. glycinea race 4 (bacterial blight of soybean) and P. s. pv. tomato DC3000 (bacterial speck of tomato). DNA sequence analysis revealed that hrpZ is the second ORF in a polycistronic operon. The amino acid sequence identities of HrpZPss/HrpZPsg and HrpZPss/HrpZPst were 79 and 63%, respectively. Although none of the HrpZ proteins showed significant overall sequence similarity with other known proteins, HrpZPst contained a 24-amino acid sequence that is homologous with a region of the PopA1 elicitor protein of the tomato pathogen, Pseudomonas solanacearum GMI1000. hrpA, the upstream ORF, was highly divergent: The amino acid sequence identities of HrpAPss/HrpAPsg and HrpAPss/HrpAPst were 91 and 28%, respectively, and no HrpA sequence showed similarity to known proteins. In contrast, the predicted products of the downstream ORFs in P. s. pv. syringae and P. s. pv. tomato, hrpB, hrpC, hrpD, and hrpE showed varying levels of similarity to those of yscI, yscJ, yscK, and yscL. These are colinearly arranged genes in the virC locus of Yersinia spp., which are involved in the secretion of the Yop virulence proteins via the type III pathway. The similarity of the Ysc proteins was generally stronger in comparisons with the P. s. pv. tomato Hrp proteins.(ABSTRACT TRUNCATED AT 250 WORDS)
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Claims 13
Claim · model-written summary
HrpZ proteins elicit the hypersensitive response in tomato but not in soybean
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- HrpZ Proteinst1
- Interactor
- Pseudomonas syringae
Pseudomonas syringaet1pathogen - Gene
- HrpZt1
- Outcome
- Hypersensitive Responset0
- Comparator
- Soybeant0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
t1“The HrpZ Proteins ofPseudomonas syringaepvs.syringae”
Claim · model-written summary
hrpZ is the second ORF in a polycistronic operon
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- hrpZe2
- Outcome
- second ORF in a polycistronic operone2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“DNA sequence analysis revealed that hrpZ is the second ORF in a polycistronic operon”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
HrpZPss and HrpZPsg share 79% amino acid sequence identity
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- HrpZPsse3
- Outcome
- amino acid sequence identities of HrpZPss/HrpZPsge3
- Value
- 79e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The amino acid sequence identities of HrpZPss/HrpZPsg and HrpZPss/HrpZPst were 79 and 63%, respectively”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
HrpZPss and HrpZPst share 63% amino acid sequence identity
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- HrpZPsse3
- Outcome
- amino acid sequence identitiese3
- Value
- 63%e3
- Qualifier
- HrpZPss/HrpZPste3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The amino acid sequence identities of HrpZPss/HrpZPsg and HrpZPss/HrpZPst were 79 and 63%, respectively”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
HrpZPst contains a 24-amino acid sequence homologous with a region of the PopA1 elicitor protein of Pseudomonas solanacearum
- Taxon
- tomatoSolanum lycopersicume4
- Interactor
- Pseudomonas solanacearume4pathogen
- Gene
- HrpZPste4
- Outcome
- homologous with a region of the PopA1 elicitor proteine4
- Value
- 24-amino acide4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“HrpZPst contained a 24-amino acid sequence that is homologous with a region of the PopA1 elicitor protein of the tomato pathogen, Pseudomonas solanacearum GMI1000”
Claim · model-written summary
hrpA is highly divergent among the P. syringae pathovars
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- hrpAe5
- Outcome
- highly divergente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“hrpA, the upstream ORF, was highly divergent: The amino acid sequence identities of HrpAPss/HrpAPsg and HrpAPss/HrpAPst were 91 and 28%, respectively, and no HrpA sequence showed similarity to known proteins”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
HrpAPss and HrpAPsg share 91% amino acid sequence identity
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- HrpAPsse5
- Outcome
- amino acid sequence identities of HrpAPss/HrpAPsge5
- Value
- 91e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“hrpA, the upstream ORF, was highly divergent: The amino acid sequence identities of HrpAPss/HrpAPsg and HrpAPss/HrpAPst were 91 and 28%, respectively, and no HrpA sequence showed similarity to known proteins”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
HrpAPss and HrpAPst share 28% amino acid sequence identity
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- HrpAPsse5
- Outcome
- amino acid sequence identitiese5
- Value
- 28%e5
- Qualifier
- HrpAPss/HrpAPste5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“hrpA, the upstream ORF, was highly divergent: The amino acid sequence identities of HrpAPss/HrpAPsg and HrpAPss/HrpAPst were 91 and 28%, respectively, and no HrpA sequence showed similarity to known proteins”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
No HrpA sequence shows similarity to known proteins
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- HrpAe5
- Outcome
- no HrpA sequence showed similarity to known proteinse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“hrpA, the upstream ORF, was highly divergent: The amino acid sequence identities of HrpAPss/HrpAPsg and HrpAPss/HrpAPst were 91 and 28%, respectively, and no HrpA sequence showed similarity to known proteins”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
Predicted products of hrpB, hrpC, hrpD and hrpE show varying levels of similarity to yscI, yscJ, yscK and yscL
- Taxon
- tomatoSolanum lycopersicume6
- Gene
- hrpB, hrpC, hrpD, and hrpEe6
- Outcome
- varying levels of similaritye6
- Comparator
- yscI, yscJ, yscK, and yscLe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“the predicted products of the downstream ORFs in P. s. pv. syringae and P. s. pv. tomato, hrpB, hrpC, hrpD, and hrpE showed varying levels of similarity to those of yscI, yscJ, yscK, and yscL”
Claim · model-written summary
Similarity of the Ysc proteins is generally stronger in comparisons with the P. s. pv. tomato Hrp proteins
- Taxon
- tomatoSolanum lycopersicume7
- Outcome
- similarity of the Ysc proteinse7
- Direction
- strongere7
- Comparator
- P. s. pv. tomato Hrp proteinse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The similarity of the Ysc proteins was generally stronger in comparisons with the P. s. pv. tomato Hrp proteins”
Claim · model-written summary
P. syringae pv. syringae 61 secretes an HR elicitor (HrpZPss) in an hrp-dependent manner
- Taxon
- TomatoSolanum lycopersicumt0
- Interactor
- P. s. pv. syringae 61e1pathogen
- Gene
- HrpZPsse1
- Outcome
- secretes an HR elicitore1
- Qualifier
- in a hrp-dependent mannere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“P. s. pv. syringae 61 secretes an HR elicitor, harpinPss (HrpZPss), in a hrp-dependent manner”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”
Claim · model-written summary
No HrpZ protein shows significant overall sequence similarity with other known proteins
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- HrpZe0
- Outcome
- none of the HrpZ proteins showed significant overall sequence similarity with other known proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although none of the HrpZ proteins showed significant overall sequence similarity with other known proteins”
t0“Elicit the Hypersensitive Response in Tomato but not Soybean”