Abstract
The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41. Approximately 18,000 plants/leaf roll were obtained in vitro in 12 weeks, when both culture stages were undertaken using temporary immersion, compared with approximately 2000 plants/leaf roll produced on semi-solid medium. However, due to hyperhydricity, only ~ 34% of the plants produced in RITA® survived acclimatization. To overcome this, and realize the potential yields of the RITA® system, various culture conditions were investigated, viz. nutrient and sucrose supplies, a rockwool substrate and the immersion regime. Of these, increasing the resting time between immersions from 1 min/12 h to 1 min/72 h, and lowering MS nutrient to 1/2 strength, proved the most beneficial, resulting in 60% acclimation success. Genetic fidelity of these plants was investigated by AFLP analyses where only 0–0.9%, of polymorphic bands were scored compared with the conventionally- propagated N41 control. Phenotypic characterization of plants grown in the field for 6 months showed that, although all in vitro derived plants had a reduced stalk diameter relative to the control, there were no significant differences regarding stalk mass, height and population.
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Claims 7
Claim · model-written summary
The RITA system was far superior to a semi-solid agar substrate for in vitro sugarcane plant yields in genotype N41.
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
Claim · model-written summary
Temporary immersion gave about 18,000 plants per leaf roll in 12 weeks, compared with about 2000 on semi-solid medium.
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
e1“Approximately 18,000 plants/leaf roll were obtained in vitro in 12 weeks, when both culture stages were undertaken using temporary immersion, compared with approximately 2000 plants/leaf roll produced on semi-solid medium”
Claim · model-written summary
Only about 34% of the plants produced in RITA survived acclimatisation because of hyperhydricity.
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
e2“However, due to hyperhydricity, only ~ 34% of the plants produced in RITA® survived acclimatization”
Claim · model-written summary
Longer resting time between immersions with half-strength MS was the most beneficial treatment, giving 60% acclimation success.
- Taxon
- sugarcanee0
- Treatment
- increasing the resting time between immersions from 1 min/12 h to 1 min/72 h, and lowering MS nutrient to 1/2 strengthe3
- Outcome
- acclimation successe3
- Direction
- most beneficiale3
- Value
- 60%e3
Not reportedComparator
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
e3“increasing the resting time between immersions from 1 min/12 h to 1 min/72 h, and lowering MS nutrient to 1/2 strength, proved the most beneficial, resulting in 60% acclimation success”
Claim · model-written summary
Plants derived from RITA showed only 0 to 0.9% polymorphic AFLP bands compared with the conventionally propagated N41 control.
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
e4“Genetic fidelity of these plants was investigated by AFLP analyses where only 0–0.9%, of polymorphic bands were scored compared with the conventionally- propagated N41 control”
Claim · model-written summary
In-vitro-derived plants had a reduced stalk diameter relative to the control when grown in the field for 6 months.
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
e5“Phenotypic characterization of plants grown in the field for 6 months showed that, although all in vitro derived plants had a reduced stalk diameter relative to the control, there were no significant differences regarding stalk mass, height and population”
Claim · model-written summary
In-vitro-derived plants showed no significant difference from the control in stalk mass, height and population after 6 months in the field.
Verbatim
e0“The effect of employing a RITA® system in one or both of somatic embryo induction and germination stages was investigated, and it was deemed far superior to a semi-solid (agar) substrate in terms of in vitro plant yields for sugarcane genotype N41”
e5“Phenotypic characterization of plants grown in the field for 6 months showed that, although all in vitro derived plants had a reduced stalk diameter relative to the control, there were no significant differences regarding stalk mass, height and population”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.