Abstract
In order to identify genes that are temporally and spatially regulated during wound response, a cDNA population in mechanically wounded tobacco leaves was screened by the fluorescence differential display method. Of 28 clones initially identified to have altered levels of transcripts within 3 h of wounding, eight were characterized. Although each clone showed a unique pattern of transcript accumulation, one distinct clone was further characterized because of its immediate-early response. Its transcripts began to accumulate 10 min after wounding, reached a maximum level within 1 h and disappeared after 2 h. The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B, indicating that cytosolic acidification could be one of the signals for immediate-early response of this gene. The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952. The putative polypeptide is rich in lysine (K), glutamic acid (E) and aspartic acid (D), which constitute up to 70% of total amino acids, and was therefore designated as KED. The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components.
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Claims 8
Claim · model-written summary
Eight of 28 candidate wound-responsive clones were characterized.
- Taxon
- tobacco
Nicotiana tabacumt0 - Outcome
- eight were characterizede0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of 28 clones initially identified to have altered levels of transcripts within 3 h of wounding, eight were characterized.”
t0“tobacco”
Claim · model-written summary
Each clone had a unique transcript accumulation pattern.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- unique pattern of transcript accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each clone showed a unique pattern of transcript accumulation”
t0“tobacco”
Claim · model-written summary
One clone accumulated transcripts at 10 minutes, peaked within one hour, and disappeared by two hours.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- transcriptse0
- Direction
- began to accumulatee0
- Value
- 10 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its transcripts began to accumulate 10 min after wounding, reached a maximum level within 1 h and disappeared after 2 h.”
t0“tobacco”
Claim · model-written summary
Propionic acid elicited the systemic early response.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- propionic acide0
- Outcome
- responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B”
t0“tobacco”
Claim · model-written summary
Erythrosin B elicited the systemic early response.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- erythrosin Be0
- Outcome
- responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B”
t0“tobacco”
Claim · model-written summary
The cDNA encoded a 513 amino acid polypeptide with relative molecular mass 60,952.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- polypeptidee0
- Value
- 513 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952.”
t0“tobacco”
Claim · model-written summary
KED had up to 70% lysine, glutamic acid and aspartic acid.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- KEDe0
- Outcome
- lysine (K), glutamic acid (E) and aspartic acid (D)e0
- Value
- 70%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The putative polypeptide is rich in lysine (K), glutamic acid (E) and aspartic acid (D), which constitute up to 70% of total amino acids, and was therefore designated as KED.”
t0“tobacco”
Claim · model-written summary
KED contained a hydrophilic N-terminal region and hydrophobic C-terminal region.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- KEDe0
- Outcome
- highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region”
t0“tobacco”