Paper · 2013 · Seed Science and Technology

Use of SSR markers to determine the genetic purity of a popular Australian wheat variety and consequences for stripe rust reactions

S. Simpfendorfer; A. Martin; M.W. Sutherland

5
claims
6
verbatim quotes
4
citations
2
research areas
—
Not open access

Abstract

Sunvale is a popular bread wheat variety grown throughout central and northern New South Wales in Australia which has moderate resistance to the fungal leaf disease stripe rust. However, significant levels of stripe rust have been reported in Sunvale crops over the past few seasons. Sunvale is a 17-year-old variety so genetic purity may also be involved in the numerous reports of increased disease susceptibility in grower crops. Twelve SSR markers were used to determine the genetic purity of 23 grower-retained seed lots of cv. Sunvale. Sixteen of the 23 grower-retained Sunvale seed lots (70%) contained at least 5% contamination from a different genotype, while seven samples (30%) contained > 10% contamination. The SSR analysis was complemented by field observations of the various seed lots in a replicated small plot trial. In 17 of the 19 grower-retained Sunvale seed lots that had some level of contamination with another genotype(s), at least one contaminant was very susceptible to stripe rust. The pure Sunvale plants in the plots showed a moderately resistant reaction. This is the first report of seed impurity being definitively determined as the cause of unexpected stripe rust responses within a wheat variety.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

Sixteen of 23 Sunvale seed lots contained at least 5% genotype contamination.

Taxon
Sunvalee0
Material
Sunvalee0
Outcome
contamination from a different genotypee0
Value
70%e0

Verbatim

e0

“Sixteen of the 23 grower-retained Sunvale seed lots (70%) contained at least 5% contamination from a different genotype”

✓ located verbatim in the source · abstract, characters 542–661 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seven Sunvale seed lots had over 10% genotype contamination.

Taxon
Sunvalee1
Outcome
contaminatione0
Value
30%e0
Qualifier
> 10%e0

Verbatim

e0

“while seven samples (30%) contained > 10% contamination.”

✓ located verbatim in the source · abstract, characters 663–719 · show in text ↑
e1

“Sunvale is a popular bread wheat variety”

✓ located verbatim in the source · abstract, characters 0–40 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

At least one contaminant was very susceptible to stripe rust in 17 of 19 contaminated lots.

Taxon
Sunvalee0
Interactor
stripe ruste0pathogen
Outcome
very susceptible to stripe ruste0
Value
17 of the 19e0

Verbatim

e0

“In 17 of the 19 grower-retained Sunvale seed lots that had some level of contamination with another genotype(s), at least one contaminant was very susceptible to stripe rust.”

✓ located verbatim in the source · abstract, characters 835–1009 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Pure Sunvale plants were moderately resistant to stripe rust.

Taxon
Sunvalee0
Material
pure Sunvalee0
Outcome
moderately resistant reactione0

Verbatim

e0

“The pure Sunvale plants in the plots showed a moderately resistant reaction.”

✓ located verbatim in the source · abstract, characters 1010–1086 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Seed impurity was identified as the cause of unexpected stripe rust responses.

Interactor
stripe ruste0pathogen
Outcome
unexpected stripe rust responsese0
Qualifier
seed impuritye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“seed impurity being definitively determined as the cause of unexpected stripe rust responses within a wheat variety”

✓ located verbatim in the source · abstract, characters 1115–1230 · show in text ↑