Paper · 1983 · Botanical Gazette

Avirulence and Reduced Extracellular Enzyme Activity in Geotrichum candidum

L. Beraha; V. Wisniewski; E. D. Garber

6
claims
6
verbatim quotes
11
citations
1
research areas
—
Not open access

Abstract

Mutagenized avirulent mutants (AV) of Geotrichum candidum and mutants of reduced virulence had lower polygalacturonase (PG), cellulase (Cx), and phosphatidase (PT) activity values than the virulent wild-type strain (G.c. met-1). Some of the AV mutants yielded reduced virulence revertants after mutagenesis. These revertants showed a substantial increase in PG, Cx, and PT activity but did not equal that of the wild-type strain Activities of PG, Cx, PT, and pectin methylesterase (PME) in rotted fruit were much higher than those in healthy fruit In vitro, G. candidum produced PG, Cx, and PT but no PME, pectic acid transeliminase, or pectin transeliminase. No heterokaryons were produced for complementing auxotrophic mutants of G. candidum or of Endomyces geotrichum or between species.

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Claims 6

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Avirulent and reduced-virulence Geotrichum candidum mutants had lower PG, cellulase and phosphatidase activity than the virulent wild type.

Taxon
Geotrichum candidumGeotrichum candidume0
Material
Mutagenized avirulent mutants (AV)e0
Interactor
Geotrichum candidumGeotrichum candidume0pathogen
Outcome
polygalacturonase (PG), cellulase (Cx), and phosphatidase (PT) activity valuese0
Direction
lowere0
Comparator
virulent wild-type straine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geotrichum candidum
Geotrichum candidum · TaxonYale Rosen from USA · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Mutagenized avirulent mutants (AV) of Geotrichum candidum and mutants of reduced virulence had lower polygalacturonase (PG), cellulase (Cx), and phosphatidase (PT) activity values than the virulent wild-type strain (G.c. met-1).”

✓ located verbatim in the source · abstract, characters 0–228 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Some avirulent mutants yielded reduced-virulence revertants after mutagenesis.

Material
AV mutantse0
Outcome
reduced virulence revertantse0

Not reportedTaxon

Verbatim

e0

“Some of the AV mutants yielded reduced virulence revertants after mutagenesis.”

✓ located verbatim in the source · abstract, characters 229–307 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Reduced-virulence revertants had increased PG, cellulase and phosphatidase activity, but levels remained below the wild type.

Material
revertantse0
Outcome
PG, Cx, and PT activitye0
Direction
increasee0
Comparator
wild-type straine0

Not reportedTaxon

Verbatim

e0

“These revertants showed a substantial increase in PG, Cx, and PT activity but did not equal that of the wild-type strain”

✓ located verbatim in the source · abstract, characters 308–428 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

PG, cellulase, phosphatidase and pectin methylesterase activities were higher in rotted fruit than healthy fruit.

Organ / stage
rotted fruite0
Outcome
Activities of PG, Cx, PT, and pectin methylesterase (PME)e0
Direction
highere0
Comparator
healthy fruite0

Not reportedTaxon

Verbatim

e0

“Activities of PG, Cx, PT, and pectin methylesterase (PME) in rotted fruit were much higher than those in healthy fruit”

✓ located verbatim in the source · abstract, characters 429–547 · show in text ↑
Observationobservedoriginal resultmixedmeasured

Claim · model-written summary

In vitro Geotrichum candidum produced PG, cellulase and phosphatidase, but no PME or pectin transeliminases.

Taxon
G. candidumGeotrichum candidume0
Outcome
PG, Cx, and PT but no PME, pectic acid transeliminase, or pectin transeliminasee0
Setting
In vitroe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geotrichum candidum
Geotrichum candidum · TaxonYale Rosen from USA · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“In vitro, G. candidum produced PG, Cx, and PT but no PME, pectic acid transeliminase, or pectin transeliminase.”

✓ located verbatim in the source · abstract, characters 548–659 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No heterokaryons formed among the tested Geotrichum candidum and Endomyces geotrichum mutants.

Taxon
G. candidumGeotrichum candidume0
Interactor
Endomyces geotrichumGeotrichum galactomycetume0other
Outcome
No heterokaryonse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geotrichum candidum
Geotrichum candidum · TaxonYale Rosen from USA · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“No heterokaryons were produced for complementing auxotrophic mutants of G. candidum or of Endomyces geotrichum or between species.”

✓ located verbatim in the source · abstract, characters 660–790 · show in text ↑