Paper · 1999 · CYTOLOGIA

A Karyotype Comparison of Nine Species of Aneuploid Zamia by Using the Conventional Orcein Staining and the Fluorochrome CMA-DAPI Differential Staining Methods.

Norikazu Tagashira; Katsuhiko Kondo

15
claims
20
verbatim quotes
8
citations
1
research areas
✓
Open access

Abstract

Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaea had the common chromosome number of 2n= 16 and the common karyotype which consisted of 12 median-, 2 submedianand 2 subterminal-centromeric chromosomes. The 4 species showed commonly the CMA-positive band in the centromeric region of 2 median-, 2 submedian-and 2 subterminal-centromeric chromosomes. In contrast, Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazquezii had commonly the chromosome number of 2n=18 and 10 median-and 2 submedian-centromeric chromosomes in karyotype but exhibited 6 different chromosomes from each other. Zamia furfuracea and Z. loddigesii displayed the CMA-positive band in the centromeric region of 8 median-and 6 acro-centromeric chromosomes. Zamia skinneri performed the CMA-positive band in the centromeric region of 4 acro-and 2 terminal-centromeric chromosomes, while Z. vazquezii performed the CMA-positive band in the centromeric region of 4 median-and 6 terminal-centromeric chromosomes. Zamia muricata had the chromosome number of 2n=23 differed from that of the previous count, the karyotype which consisted of 7 median-and 16 acro-centromeric chromosomes and the CMA-positive band in the centromeric region of 4 median-and 16 acro-centromeric chromosomes. Then, the 9 species of Zamia studied exhibited unclear DAPI-positive bands near the centromeric region of most of their chromosomes. By comparison, the closely related Ceratozamia mexicana had the chromosome number of 2n=16 and the CMA-positive band at the terminal region of 6 chromosomes.

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Claims 15

Observationobservedoriginal resultmeasured

Claim · model-written summary

Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaea had the common chromosome number 2n=16.

Taxon
Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaeaZamia pumilaZamia integrifoliaZamia pygmaeaZamia angustifoliae1
Outcome
chromosome numbere1
Value
2n= 16e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia pumila
Zamia pumila · TaxonPublic domain · Wikimedia Commons ↗
Zamia integrifolia
Zamia integrifolia · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia pygmaea
Zamia pygmaea · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗
Zamia angustifolia
Zamia angustifolia · TaxonStan Shebs · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaea had the common chromosome number of 2n= 16”

✓ located verbatim in the source · abstract, characters 0–104 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The four 2n=16 Zamia species shared a karyotype of 12 median-, 2 submedian- and 2 subterminal-centromeric chromosomes.

Taxon
Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaeaZamia pumilaZamia integrifoliaZamia pygmaeaZamia angustifoliae0
Outcome
karyotypee1
Value
12 median-, 2 submedianand 2 subterminal-centromeric chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia pumila
Zamia pumila · TaxonPublic domain · Wikimedia Commons ↗
Zamia integrifolia
Zamia integrifolia · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia pygmaea
Zamia pygmaea · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗
Zamia angustifolia
Zamia angustifolia · TaxonStan Shebs · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaea”

✓ located verbatim in the source · abstract, characters 0–61 · show in text ↑
e1

“the common karyotype which consisted of 12 median-, 2 submedianand 2 subterminal-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 109–213 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The four 2n=16 Zamia species commonly showed a CMA-positive band in the centromeric region of 2 median-, 2 submedian- and 2 subterminal-centromeric chromosomes.

Taxon
Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaeaZamia pumilaZamia integrifoliaZamia pygmaeaZamia angustifoliae0
Outcome
CMA-positive bande1
Value
2 median-, 2 submedian-and 2 subterminal-centromeric chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia pumila
Zamia pumila · TaxonPublic domain · Wikimedia Commons ↗
Zamia integrifolia
Zamia integrifolia · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia pygmaea
Zamia pygmaea · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗
Zamia angustifolia
Zamia angustifolia · TaxonStan Shebs · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Zamia angustifolia, Z. integrifolia, Z. pumila and Z. pygmaea”

✓ located verbatim in the source · abstract, characters 0–61 · show in text ↑
e1

“The 4 species showed commonly the CMA-positive band in the centromeric region of 2 median-, 2 submedian-and 2 subterminal-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 215–360 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazquezii had the common chromosome number 2n=18.

Taxon
Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazqueziiZamia furfuraceaZamia loddigesiiZamia skinneriZamia vazqueziie1
Outcome
chromosome numbere1
Value
2n=18e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia furfuracea
Zamia furfuracea · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia loddigesii
Zamia loddigesii · TaxonSarah Stierch · CC BY 4.0 · Wikimedia Commons ↗
Zamia skinneri
Zamia skinneri · TaxonC T Johansson · CC BY-SA 4.0 · Wikimedia Commons ↗
Zamia vazquezii
Zamia vazquezii · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazquezii had commonly the chromosome number of 2n=18 and 10 median-and 2 submedian-centromeric chromosomes in karyotype but exhibited 6 different chromosomes from each other”

✓ located verbatim in the source · abstract, characters 375–598 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

These four 2n=18 Zamia species commonly had a karyotype of 10 median- and 2 submedian-centromeric chromosomes.

Taxon
Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazqueziiZamia furfuraceaZamia loddigesiiZamia skinneriZamia vazqueziie1
Outcome
karyotypee1
Value
10 median-and 2 submedian-centromeric chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia furfuracea
Zamia furfuracea · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia loddigesii
Zamia loddigesii · TaxonSarah Stierch · CC BY 4.0 · Wikimedia Commons ↗
Zamia skinneri
Zamia skinneri · TaxonC T Johansson · CC BY-SA 4.0 · Wikimedia Commons ↗
Zamia vazquezii
Zamia vazquezii · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazquezii had commonly the chromosome number of 2n=18 and 10 median-and 2 submedian-centromeric chromosomes in karyotype but exhibited 6 different chromosomes from each other”

✓ located verbatim in the source · abstract, characters 375–598 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

These four 2n=18 Zamia species differed from each other in 6 chromosomes.

Taxon
Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazqueziiZamia furfuraceaZamia loddigesiiZamia skinneriZamia vazqueziie1
Outcome
different chromosomese1
Value
6 different chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia furfuracea
Zamia furfuracea · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia loddigesii
Zamia loddigesii · TaxonSarah Stierch · CC BY 4.0 · Wikimedia Commons ↗
Zamia skinneri
Zamia skinneri · TaxonC T Johansson · CC BY-SA 4.0 · Wikimedia Commons ↗
Zamia vazquezii
Zamia vazquezii · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Z. furfuracea, Z. loddigesii, Z. skinneri and Z. vazquezii had commonly the chromosome number of 2n=18 and 10 median-and 2 submedian-centromeric chromosomes in karyotype but exhibited 6 different chromosomes from each other”

✓ located verbatim in the source · abstract, characters 375–598 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zamia furfuracea and Z. loddigesii displayed the CMA-positive band in the centromeric region of 8 median- and 6 acro-centromeric chromosomes.

Taxon
Zamia furfuracea and Z. loddigesiiZamia furfuraceaZamia loddigesiie1
Outcome
CMA-positive bande1
Value
8 median-and 6 acro-centromeric chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia furfuracea
Zamia furfuracea · Taxon · picture from its Wikimedia Commons categoryForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗
Zamia loddigesii
Zamia loddigesii · TaxonSarah Stierch · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Zamia furfuracea and Z. loddigesii displayed the CMA-positive band in the centromeric region of 8 median-and 6 acro-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 600–739 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zamia skinneri showed the CMA-positive band in the centromeric region of 4 acro- and 2 terminal-centromeric chromosomes.

Taxon
Zamia skinneriZamia skinnerie1
Outcome
CMA-positive bande1
Value
4 acro-and 2 terminal-centromeric chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia skinneri
Zamia skinneri · TaxonC T Johansson · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Zamia skinneri performed the CMA-positive band in the centromeric region of 4 acro-and 2 terminal-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 741–862 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Z. vazquezii showed the CMA-positive band in the centromeric region of 4 median- and 6 terminal-centromeric chromosomes.

Taxon
Z. vazqueziiZamia vazqueziie1
Outcome
CMA-positive bande1
Value
4 median-and 6 terminal-centromeric chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia vazquezii
Zamia vazquezii · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Z. vazquezii performed the CMA-positive band in the centromeric region of 4 median-and 6 terminal-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 870–991 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zamia muricata had the chromosome number 2n=23, which differed from the previous count.

Taxon
Zamia muricataZamia muricatae1
Outcome
chromosome numbere1
Value
2n=23e1

Verbatim

e1

“Zamia muricata had the chromosome number of 2n=23 differed from that of the previous count”

✓ located verbatim in the source · abstract, characters 993–1083 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zamia muricata had a karyotype of 7 median- and 16 acro-centromeric chromosomes.

Taxon
Zamia muricataZamia muricatae0
Outcome
karyotypee1
Value
7 median-and 16 acro-centromeric chromosomese1

Verbatim

e0

“Zamia muricata”

✓ located verbatim in the source · abstract, characters 993–1007 · show in text ↑
e1

“the karyotype which consisted of 7 median-and 16 acro-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 1085–1162 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zamia muricata showed the CMA-positive band in the centromeric region of 4 median- and 16 acro-centromeric chromosomes.

Taxon
Zamia muricataZamia muricatae0
Outcome
CMA-positive bande1
Value
4 median-and 16 acro-centromeric chromosomese1

Verbatim

e0

“Zamia muricata”

✓ located verbatim in the source · abstract, characters 993–1007 · show in text ↑
e1

“the CMA-positive band in the centromeric region of 4 median-and 16 acro-centromeric chromosomes”

✓ located verbatim in the source · abstract, characters 1167–1262 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The nine Zamia species studied exhibited unclear DAPI-positive bands near the centromeric region of most of their chromosomes.

Taxon
ZamiaZamiae1
Outcome
unclear DAPI-positive bandse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zamia
Zamia · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“9 species of Zamia studied exhibited unclear DAPI-positive bands near the centromeric region of most of their chromosomes”

✓ located verbatim in the source · abstract, characters 1274–1395 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ceratozamia mexicana, closely related to Zamia, had the chromosome number 2n=16.

Taxon
Ceratozamia mexicanaCeratozamia mexicanae1
Outcome
chromosome numbere1
Value
2n=16e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ceratozamia mexicana
Ceratozamia mexicana · TaxonPeregrinusX · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the closely related Ceratozamia mexicana had the chromosome number of 2n=16”

✓ located verbatim in the source · abstract, characters 1412–1487 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ceratozamia mexicana showed the CMA-positive band at the terminal region of 6 chromosomes.

Taxon
Ceratozamia mexicanaCeratozamia mexicanae0
Outcome
CMA-positive bande1
Value
6 chromosomese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ceratozamia mexicana
Ceratozamia mexicana · TaxonPeregrinusX · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Ceratozamia mexicana”

✓ located verbatim in the source · abstract, characters 1432–1452 · show in text ↑
e1

“the CMA-positive band at the terminal region of 6 chromosomes”

✓ located verbatim in the source · abstract, characters 1492–1553 · show in text ↑