Abstract
The regulatory photoreceptor, phytochrome, controls the expression of numerous genes, including its own phyA genes, which are transcriptionally repressed in response to light. Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression. Partial complementary DNA clones have been obtained for a rice nuclear protein, designated GT-2, that binds in a highly sequence-specific fashion to this motif. Mutational analysis shows that the paired G's are most crucial to binding. GT-2 has domains related to certain other transcription factors. Northern blot analysis shows that GT-2 messenger RNA levels decline in white light although red and far red light pulses are ineffective.
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Claims 8
Claim · model-written summary
A GT motif is critical for rice phyA promoter expression.
- Taxon
- rice
Oryza sativae0 - Gene
- GT motif, GCGGTAATTe0
- Outcome
- critical for expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
Claim · model-written summary
Partial cDNA clones were obtained for rice GT-2.
- Taxon
- riceOryza sativae0
- Gene
- GT-2e0
- Outcome
- Partial complementary DNA clonese0
- Value
- Partiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Partial complementary DNA clones have been obtained for a rice nuclear protein, designated GT-2”
Claim · model-written summary
GT-2 binds the motif with high sequence specificity.
- Taxon
- riceOryza sativae1
- Gene
- GT-2e0
- Outcome
- binds in a highly sequence-specific fashione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GT-2, that binds in a highly sequence-specific fashion to this motif”
t0“Phytochrome Gene Promoter”
e1“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
Claim · model-written summary
Paired G bases are most crucial for GT-2 binding.
- Taxon
- riceOryza sativae1
- Outcome
- paired G's are most crucial to bindinge0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutational analysis shows that the paired G's are most crucial to binding”
t0“Phytochrome Gene Promoter”
e1“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
Claim · model-written summary
GT-2 has domains related to other transcription factors.
- Taxon
- riceOryza sativae1
- Gene
- GT-2e0
- Outcome
- domains related to certain other transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GT-2 has domains related to certain other transcription factors”
t0“Phytochrome Gene Promoter”
e1“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
Claim · model-written summary
GT-2 messenger RNA levels decline in white light.
- Taxon
- riceOryza sativae1
- Treatment
- white lighte0
- Gene
- GT-2e0
- Outcome
- messenger RNA levelse0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GT-2 messenger RNA levels decline in white light although red and far red light pulses are ineffective”
t0“Phytochrome Gene Promoter”
e1“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
Claim · model-written summary
Red light pulses were ineffective for GT-2 mRNA levels.
- Taxon
- riceOryza sativae1
- Treatment
- rede0
- Outcome
- GT-2 messenger RNA levelse0
- Direction
- ineffectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GT-2 messenger RNA levels decline in white light although red and far red light pulses are ineffective”
t0“Phytochrome Gene Promoter”
e1“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
Claim · model-written summary
Far red light pulses were ineffective for GT-2 mRNA levels.
- Taxon
- riceOryza sativae1
- Treatment
- far red light pulsese0
- Outcome
- GT-2 messenger RNA levelse0
- Direction
- ineffectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GT-2 messenger RNA levels decline in white light although red and far red light pulses are ineffective”
t0“Phytochrome Gene Promoter”
e1“Functional analysis of a rice phyA gene promoter, by means of microprojectile-mediated gene transfer, indicates that a GT motif, GCGGTAATT, closely related to elements in the promoters of a number of other light-regulated genes, is critical for expression”
The same questions in other papers
light · molecular: metabolites · Oryza sativa (rice)Positive and negative results
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See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.