Abstract
Abstract Analyses, based on the structure of the rDNA region, were developed to examine the possibility of identifying and typing some of the most important Tuber spp at both the symbiotic and non‐symbiotic levels. Internal transcribed spacers (ITS) were amplified by the polymerase chain reaction using specific primer pairs on genomic DNAs, isolated from ascocarps and ectomycorrhizae of various Tuber spp and from rootlets of uninoculated host plants. The variability in the lengths of ITS amplification products of DNA isolated from fruit bodies allowed a primary discrimination of the Tuber spp considered. At the ecto‐mycorrhizal level the same species showed a pattern which was the combination of those typical of Tuber plus the host plants. Southern analyses, performed using the ITS product of Tuber brumale as a probe, confirmed the previous results: each fungus species showed a specific pattern, as well as each Tuber ‐host plant combination. It is concluded that molecular markers from the rDNA region can be used to differentiate Tuber spp and that the method proposed could be used to check and certify mycorrhizally infected plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
ITS product lengths distinguished the Tuber species studied using fruit-body DNA.
TuberOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The variability in the lengths of ITS amplification products of DNA isolated from fruit bodies allowed a primary discrimination of the Tuber spp considered”
Claim · model-written summary
Ectomycorrhizal ITS patterns combined Tuber and host plant patterns.
- Taxon
- host plantse0
- Outcome
- pattern which was the combination of those typical of Tuber plus the host plantse0
- Comparator
- those typical of Tubere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the ecto‐mycorrhizal level the same species showed a pattern which was the combination of those typical of Tuber plus the host plants”
Claim · model-written summary
Southern analysis found species-specific fungus patterns and distinct Tuber–host plant combinations.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Southern analyses, performed using the ITS product of Tuber brumale as a probe, confirmed the previous results: each fungus species showed a specific pattern, as well as each Tuber ‐host plant combination”
Claim · model-written summary
The authors concluded rDNA markers can differentiate Tuber species and certify mycorrhizally infected plants.
- Taxon
- plantsViridiplantaee0
- Outcome
- check and certify mycorrhizally infected plantse0
- Comparator
- differentiate Tuber sppe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“molecular markers from the rDNA region can be used to differentiate Tuber spp and that the method proposed could be used to check and certify mycorrhizally infected plants”