Abstract
Abstract Tomato aspermy virus (TAV), an important viral pathogen of chrysanthemums, was detected by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) from chrysanthemums exhibiting mottling and deformed inflorescence grown in various states of India. Out of 15 cultivars (cvs.) tested, 11 (73.3%) were found to be positive for TAV. On sap inoculation the virus produced local lesions on Chenopodium spp. and Cucumis sativus. Nicotiana clevelandii, N. glutinosa, N. megalosiphon and N. tabacum reacted systemically to the virus producing severe mosaic, leaf deformation and characteristic leaf enations. Tomato plants produced malformed fruits (2-3/plant) with a few seeds. Myzus persicae and Aphis gossypii transmitted the virus non-persistently. Electron microscopy of partially purified viral preparations revealed polyhedral virions (ca. 29 nm dia). Cytopathology of infected leaves of N. clevelandii showed crystalline inclusions containing virions in the central vacuole of infected cells. The virions showed very good clumping with antiserum to TAV in liquid phase immuno-electron microscopy. Slot blot hybridization was performed to detect the virus in various chrysanthemum cultivars. The virus positivity was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing of coat protein gene (CP). The amplified 657 bp fragment was about 97% identical with respect to CP sequences of other TAV isolates available in the database. In terms of derived amino acid sequence similarity, the homology value was 99%. In order to amplify RNA 1, RNA 2 and RNA 3, multiplex RT-PCR was performed. High genetic similarities of CP gene of TAV Indian isolates with that of other isolates of TAV indicate their probable common ancestry.
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Claims 15
Claim · model-written summary
TAV was detected in symptomatic chrysanthemums grown across India.
- Taxon
- chrysanthemums
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Chrysanthemume0 - Interactor
- Tomato aspermy virusTomato aspermy viruse0pathogen
- Outcome
- TAVe0
- Setting
- Indiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“Tomato aspermy virus (TAV), an important viral pathogen of chrysanthemums, was detected by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) from chrysanthemums exhibiting mottling and deformed inflorescence grown in various states of India.”
Claim · model-written summary
Eleven of 15 chrysanthemum cultivars tested positive for TAV.
- Taxon
- chrysanthemumsChrysanthemumt0
- Interactor
- TAVTomato aspermy viruse0pathogen
- Outcome
- positive for TAVe0
- Value
- 11 (73.3%)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of 15 cultivars (cvs.) tested, 11 (73.3%) were found to be positive for TAV.”
t0“chrysanthemums”
Claim · model-written summary
Sap inoculation produced local lesions on Chenopodium spp.
- Taxon
- Chenopodium spp.
Chenopodiume0 - Treatment
- sap inoculatione0
- Interactor
- viruse0pathogen
- Outcome
- local lesionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On sap inoculation the virus produced local lesions on Chenopodium spp. and Cucumis sativus.”
e1“Tomato aspermy virus (TAV)”
Claim · model-written summary
Sap inoculation produced local lesions on Cucumis sativus.
- Taxon
- Cucumis sativus
Cucumis sativuse0 - Treatment
- sap inoculatione0
- Interactor
- viruse0pathogen
- Outcome
- local lesionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On sap inoculation the virus produced local lesions on Chenopodium spp. and Cucumis sativus.”
Claim · model-written summary
Four Nicotiana hosts reacted systemically with severe mosaic, leaf deformation and enations.
- Taxon
- Nicotiana clevelandii, N. glutinosa, N. megalosiphon and N. tabacum
Nicotiana tabacum
Nicotiana glutinosa
Nicotiana clevelandii
Nicotiana megalosiphone0 - Interactor
- viruse0pathogen
- Outcome
- severe mosaic, leaf deformation and characteristic leaf enationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana clevelandii, N. glutinosa, N. megalosiphon and N. tabacum reacted systemically to the virus producing severe mosaic, leaf deformation and characteristic leaf enations.”
Claim · model-written summary
Infected tomato plants produced malformed fruits with few seeds.
- Taxon
- Tomato
Solanum lycopersicume0 - Organ / stage
- fruitse0
- Outcome
- malformed fruitse0
- Value
- 2-3/plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants produced malformed fruits (2-3/plant) with a few seeds.”
Claim · model-written summary
Myzus persicae transmitted the virus non-persistently.
- Taxon
- chrysanthemumsChrysanthemumt0
- Interactor
- Myzus persicae
Myzus persicaee0pest - Outcome
- transmitted the virus non-persistentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Myzus persicae and Aphis gossypii transmitted the virus non-persistently.”
t0“chrysanthemums”
Claim · model-written summary
Aphis gossypii transmitted the virus non-persistently.
- Taxon
- chrysanthemumsChrysanthemumt0
- Interactor
- Aphis gossypii
Aphis gossypiie0pest - Outcome
- transmitted the virus non-persistentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Myzus persicae and Aphis gossypii transmitted the virus non-persistently.”
t0“chrysanthemums”
Claim · model-written summary
Electron microscopy revealed polyhedral virions about 29 nm in diameter.
- Taxon
- chrysanthemumsChrysanthemumt0
- Outcome
- polyhedral virionse0
- Value
- ca. 29 nm diae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electron microscopy of partially purified viral preparations revealed polyhedral virions (ca. 29 nm dia).”
t0“chrysanthemums”
Claim · model-written summary
Infected N. clevelandii cells contained crystalline inclusions with virions in the central vacuole.
- Taxon
- N. clevelandiiNicotiana clevelandiie0
- Organ / stage
- leavese0
- Outcome
- crystalline inclusions containing virions in the central vacuolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytopathology of infected leaves of N. clevelandii showed crystalline inclusions containing virions in the central vacuole of infected cells.”
Claim · model-written summary
Virions clumped strongly with TAV antiserum in immuno-electron microscopy.
- Taxon
- chrysanthemumsChrysanthemumt0
- Outcome
- very good clumping with antiserum to TAVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virions showed very good clumping with antiserum to TAV in liquid phase immuno-electron microscopy.”
t0“chrysanthemums”
Claim · model-written summary
RT-PCR and coat protein sequencing confirmed TAV positivity in chrysanthemum cultivars.
- Taxon
- chrysanthemumChrysanthemume1
- Gene
- coat protein gene (CP)e0
- Outcome
- virus positivitye0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virus positivity was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing of coat protein gene (CP).”
e1“various chrysanthemum cultivars”
Claim · model-written summary
The amplified TAV coat protein fragment was about 97% identical to other isolates.
- Taxon
- chrysanthemumsChrysanthemumt0
- Gene
- CPe0
- Outcome
- identical with respect to CP sequencese0
- Value
- about 97%e0
- Comparator
- other TAV isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amplified 657 bp fragment was about 97% identical with respect to CP sequences of other TAV isolates available in the database.”
t0“chrysanthemums”
Claim · model-written summary
Derived amino acid sequences had 99% homology.
- Taxon
- chrysanthemumsChrysanthemumt0
- Outcome
- derived amino acid sequence similaritye0
- Value
- 99%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In terms of derived amino acid sequence similarity, the homology value was 99%.”
t0“chrysanthemums”
Claim · model-written summary
High coat protein gene similarity indicates probable common ancestry of Indian and other TAV isolates.
- Taxon
- chrysanthemumsChrysanthemumt0
- Gene
- CP genee0
- Outcome
- probable common ancestrye0
- Comparator
- other isolates of TAVe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High genetic similarities of CP gene of TAV Indian isolates with that of other isolates of TAV indicate their probable common ancestry.”
t0“chrysanthemums”
The same questions in other papers
inoculation · disease · Cucumis sativus (cucumber)Positive and negative results
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- positive
- positive
- positive
79 papers more in this group
See all 83 papers →inoculation · disease · ChenopodiumResults agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.