Abstract
The development of selectable markers for transformation has been a major factor in the successful genetic manipulation of plants. A new selectable marker system has been developed based on bacterial gentamicin-3-N-acetyltransferases [AAC(3)]. These enzymes inactivate aminoglycoside antibiotics by acetylation. Two examples of AAC(3) enzymes have been manipulated to be expressed in plants. Chimeric AAC(3)-III and AAC(3)-IV genes were assembled using the constitutively expressed cauliflower mosaic virus 35S promoter and the nopaline synthase 3' nontranslated region. These chimeric genes were engineered into vectors for Agrobacterium-mediated plant transformation. Petunia hybrida and Arabidopsis thaliana tissue transformed with these vectors grew in the presence of normally lethal levels of gentamicin. The transformed nature of regenerated Arabidopsis plants was confirmed by DNA hybridization analysis and inheritance of the selectable phenotype in progeny. The chimeric AAC(3)-IV gene has also been used to select transformants in several additional plant species. These results show that the bacterial AAC(3) genes will serve as useful selectable markers in plant tissue culture.
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Claims 3
Claim · model-written summary
Transformed Petunia hybrida and Arabidopsis thaliana tissue grew at normally lethal gentamicin levels.
- Taxon
- Petunia hybrida and Arabidopsis thaliana
Arabidopsis thaliana
Petunia ×hybridae0 - Treatment
- gentamicine0
- Outcome
- grew in the presence of normally lethal levels of gentamicine0
- Value
- normally lethal levels of gentamicine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Petunia hybrida and Arabidopsis thaliana tissue transformed with these vectors grew in the presence of normally lethal levels of gentamicin”
Claim · model-written summary
DNA hybridization and inherited selectable phenotype confirmed transformed regenerated Arabidopsis plants.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- transformed nature of regenerated Arabidopsis plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transformed nature of regenerated Arabidopsis plants was confirmed by DNA hybridization analysis and inheritance of the selectable phenotype in progeny”
Claim · model-written summary
The chimeric AAC(3)-IV gene selected transformants in additional plant species.
Verbatim
e0“The chimeric AAC(3)-IV gene has also been used to select transformants in several additional plant species”