Abstract
An efficient somatic embryogenesis protocol for plantlet regeneration of the commercially valuable, and nutritionally important, crop guava ( Psidium guajava L.) has been established. The immature zygotic embryos were targeted as an explant source for inducing somatic embryogenesis and plant regeneration, using four commercial cultivars (Allahabad Safeda, Lalit, Sardar and Shweta). The plantlets regenerated were assessed for genetic fidelity using random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and simple sequence repeat (SSR) molecular markers. A total of 2171 scorable bands were obtained using RAPD, ISSR or SSR, ranging from 300–3500, 250–3000 and 150–280 bp, respectively. No polymorphism was observed among the somatic embryogenesis regenerated plants, compared with respective donor mother plants. The profiles generated based on the three marker systems were found to be highly uniform and approximately 99% bands were monomorphic. This high degree of genetic uniformity (as assessed using the markers) in the somatic embryogenesis regenerated plants indicates genomic stability was maintained through the regeneration protocol. The somatic embryogenesis regenerated plants were hardened and transferred to the field for acclimatization, of which 80% plantlets survived, with all being phenotypically similar to the donor mother plants. We conclude that the RAPD, ISSR and SSR markers were informative and potentially useful in confirming the uniformity assessment of somatic embryogenesis regenerated plants.
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Claims 6
Claim · model-written summary
A somatic embryogenesis protocol regenerated guava plantlets.
- Taxon
- guava
Psidium guajavae0 - Outcome
- plantlet regeneratione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient somatic embryogenesis protocol for plantlet regeneration of the commercially valuable, and nutritionally important, crop guava ( Psidium guajava L.) has been established.”
Claim · model-written summary
Marker analysis found no polymorphism among regenerated guava plants relative to donor plants.
- Taxon
- guavaPsidium guajavat0
- Outcome
- polymorphisme0
- Direction
- No polymorphisme0
- Comparator
- respective donor mother plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No polymorphism was observed among the somatic embryogenesis regenerated plants, compared with respective donor mother plants.”
t0“guava ( Psidium guajava L.)”
Claim · model-written summary
Approximately 99% of bands were monomorphic in the three marker profiles.
- Taxon
- guavaPsidium guajavat0
- Outcome
- bands were monomorphice0
- Value
- approximately 99%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The profiles generated based on the three marker systems were found to be highly uniform and approximately 99% bands were monomorphic.”
t0“guava ( Psidium guajava L.)”
Claim · model-written summary
Eighty percent of regenerated guava plantlets survived field acclimatization.
- Taxon
- guavaPsidium guajavat0
- Outcome
- plantlets survivede0
- Value
- 80%e0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The somatic embryogenesis regenerated plants were hardened and transferred to the field for acclimatization, of which 80% plantlets survived, with all being phenotypically similar to the donor mother plants.”
t0“guava ( Psidium guajava L.)”
Claim · model-written summary
Surviving guava plantlets were phenotypically similar to donor plants.
- Taxon
- guavaPsidium guajavat0
- Outcome
- phenotypically similare0
- Comparator
- donor mother plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all being phenotypically similar to the donor mother plants”
t0“guava ( Psidium guajava L.)”
Claim · model-written summary
RAPD, ISSR and SSR markers were judged useful for confirming uniformity.
- Taxon
- guavaPsidium guajavat0
- Outcome
- uniformity assessmente0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We conclude that the RAPD, ISSR and SSR markers were informative and potentially useful in confirming the uniformity assessment of somatic embryogenesis regenerated plants.”
t0“guava ( Psidium guajava L.)”