Abstract
Isolates in the Fusarium oxysporum species complex (FOSC) from soybean range from nonpathogenic to aggressive pathogens causing seedling damping-off, wilt, and root rot. The objective of this research was to characterize the genotype and phenotype of isolates within the FOSC recovered predominantly from soybean roots and seedlings. Sequence analyses of the translation elongation factor (tef1α) gene and the mitochondrial small subunit (mtSSU), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis of the intergenic spacer (IGS) region, and identification of the mating type loci were conducted for 170 isolates. Vegetative compatibility (VC) tests were conducted for 114 isolates. Isolate aggressiveness was tested using a rolled towel assay for 159 isolates. Phylogenetic analysis of the tef1α and mtSSU and PCR-RFLP analysis of the IGS region separated the FOSC isolates into five clades, including F. commune. Both mating type loci, MAT1-1 or MAT1-2, were present in isolates from all clades. The VC tests were not informative, because most VC groups consisted of a single isolate. Isolate aggressiveness varied within and among clades; isolates in clade 2 were significantly less aggressive (P < 0.0001) when compared with isolates from the other clades and F. commune. The results from this study demonstrate the high levels of genotypic and phenotypic diversity within the FOSC from soybean but further work is needed to identify characteristics associated with pathogenic capabilities.
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Claims 6
Claim · model-written summary
Molecular analyses separated Fusarium isolates from soybean into five clades including F. commune.
- Taxon
- soybean
Glycine maxe1 - Interactor
- Fusarium oxysporum species complexFusarium oxysporum species complexe1pathogen
- Outcome
- FOSC isolates into five cladese0
- Value
- five cladese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis of the tef1α and mtSSU and PCR-RFLP analysis of the IGS region separated the FOSC isolates into five clades, including F. commune”
e1“Fusarium oxysporum species complex (FOSC) from soybean”
Claim · model-written summary
Both mating type loci occurred in isolates from all clades.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Fusarium oxysporum species complexFusarium oxysporum species complexe1pathogen
- Gene
- MAT1-1 or MAT1-2e0
- Outcome
- present in isolates from all cladese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both mating type loci, MAT1-1 or MAT1-2, were present in isolates from all clades”
e1“Fusarium oxysporum species complex (FOSC) from soybean”
Claim · model-written summary
Vegetative compatibility tests were uninformative because most groups contained one isolate.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Fusarium oxysporum species complexFusarium oxysporum species complexe1pathogen
- Outcome
- not informativee0
- Value
- a single isolatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The VC tests were not informative, because most VC groups consisted of a single isolate”
e1“Fusarium oxysporum species complex (FOSC) from soybean”
Claim · model-written summary
Fusarium isolate aggressiveness on soybean varied within and among clades.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Fusarium oxysporum species complexFusarium oxysporum species complexe1pathogen
- Outcome
- Isolate aggressivenesse0
- Direction
- variede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolate aggressiveness varied within and among clades”
e1“Fusarium oxysporum species complex (FOSC) from soybean”
Claim · model-written summary
Clade 2 isolates were significantly less aggressive than isolates in other clades and F. commune.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Fusarium oxysporum species complexFusarium oxysporum species complexe1pathogen
- Outcome
- isolates in clade 2 were significantly less aggressivee0
- Direction
- less aggressivee0
- Comparator
- isolates from the other clades and F. communee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolates in clade 2 were significantly less aggressive (P < 0.0001) when compared with isolates from the other clades and F. commune”
e1“Fusarium oxysporum species complex (FOSC) from soybean”
Claim · model-written summary
The Fusarium complex from soybean showed high genotypic and phenotypic diversity.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Fusarium oxysporum species complexFusarium oxysporum species complexe1pathogen
- Outcome
- genotypic and phenotypic diversitye0
- Direction
- high levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high levels of genotypic and phenotypic diversity within the FOSC from soybean”
e1“Fusarium oxysporum species complex (FOSC) from soybean”